CHEMOPREVENTIVE ISOFLAVONES FROM MICROORGANISMS

类黄酮是一种植物物质，能赋予花朵和水果颜色， 而且它们也包括在正常的饮食中。异黄酮类是一类 大豆中发现的类黄酮类化合物。流行病学研究表明，在 饮食中富含大豆的地理区域减少了 乳腺癌发病率金雀异黄素是一种异黄酮，其存在于 大豆被认为与化学预防作用有关。金雀异黄素是 也是拓扑异构酶II和蛋白酪氨酸激酶的抑制剂。 因为这两种酶参与了细胞分化和 此外，金雀异黄素还可能具有化疗作用。 目前对染料木素的生物医学研究受到抑制 人工合成或提取纯化合物的高成本 来自大豆。据报道，微生物也能产生 染料木素，可以为该化合物提供一个经济的来源。 微生物发酵的特点是易于加工。 通过遗传和生理操作进行改良。同相 我们建议确认异黄酮类的微生物生产，并开始 开发遗传和生化分析所需的工具 异黄酮类生产。在第二阶段，我们希望创造一个高效的 发酵或微生物转化过程及使用靶向 遗传学将产生用于生物测试的新的异黄酮类化合物。

Flavonoids ar plant substances that give flowers and fruits their color, and they are included in a normal diet. The isoflavones are a class of flavonoids found in soybean. Epidemiological studies have shown that in geographic areas where diets are rich in soybeans there is a reduced incidence of breast cancer Genistein is an isoflavone whose presence in soybeans has been linked to the chemopreventive effect. Genistein is also an inhibitor of topoisomerase II and protein tyrosine kinase. Because these two enzymes are involved in cellular differentiation and proliferation, genistein could also have chemotherapeutic properties. Much needed biomedical research on geneistein is currently inhibited by the high cost of the pure compound synthetically produced or extracted from soybeans. Microorganisms have also been reported to produce genistein and could provide an economical source for the compound. Microbial fermentations are characteristically amenable to process improvement through genetic and physiological manipulations. In phase one we propose to confirm microbial production of isoflavones and begin to develop the tools needed for genetic and biochemical analysis of isoflavone production. In phase 2, we hope to create an efficient fermentation or microbial transformation process and use targeted genetics to produce new isoflavone analogs for biological testing.