SOUTHERN CALIFORNIA AIDS MALIGNANCY NETWORK

加州大学洛杉矶分校和南加州大学艾滋病项目将标本储存库作为资源 为各自的研究界建立实验室。因为 每个储存库的独特资源，重要的外部 合作是存在的。这些实验室的设计是为了支持临床 试验以及基础科学和流行病学调查 艾滋病相关恶性肿瘤的发病机制。锡达斯西奈医疗中心是 以社区为基础的三级护理医院，作为转诊中心 在洛杉矶进行与艾滋病相关的淋巴瘤病理研究。把这些放在一起 三个中心获得了200多例新的卡波西肉瘤病例和100例 每年都会得淋巴瘤。 在加州大学洛杉矶分校，该实验室拥有超过15,000份计算机库存 血清样本、600份血浆样本和800份冷冻活淋巴细胞 准备工作。该银行提供卡波西肉瘤细胞和艾滋病的分离株 用于基础科学研究的淋巴瘤细胞。样本链接到 参与临床的临床状态和结果 审判。大多数患者都有超过5年的多个样本， 纵向数据。在南加州大学，实验室储存了5000多个血清样本， 306份尿样，84份卡波西肉瘤病变的RNA/组织标本， 和20多个新鲜的卡波西肉瘤细胞系。大部分样品 收集自一项广泛的流行病学调查，旨在 阐明导致卡波西病发生的病原体或过程 肉瘤或来自抗KS和抗淋巴瘤药物的临床试验。 合作者使用这些样本排除了罗克利姆病毒是一种致病因素 KS试剂，检查细胞因子扰动与KS的关系 淋巴瘤的发生，识别受体后的关键分子 KS中导致增殖的信号转导途径，演示 淋巴瘤中重链的利用是非随机的，调查 艾滋病恶性肿瘤的克隆性，以及分离的A基因片段 可能与经典和艾滋病相关的KS有关的有机体。在雪松 西奈半岛，超过235例艾滋病相关淋巴瘤被分析为 约会。这些研究包括广泛的免疫表型特征， EBV研究和P53突变的描绘。到目前为止，这三人 资料库为多个机构的25名调查人员提供服务 关于需要临床材料的广泛的基础科学问题。 这些银行提供稀有标本的独特能力(这样的雌性 或HIV阴性的流行KS细胞株)和相关的临床 将结果放在上下文中所需的信息是最多的 这个储存库的优势。 此应用程序用于联合存储库，以存储与以下内容相关的样本 加州大学洛杉矶分校在当地处理后与艾滋病相关的恶性肿瘤。这个 应用程序融合了每个示例的各自优势 拥有成功的艾滋病癌症患者天赋的资料库 调查人员。该银行已经包含了大量对 卡波西肉瘤与淋巴瘤发病机制的研究。有价值的控制权 来自匹配的基于社区的控件的样本是独一无二的 存储库。合并后的存储库将继续收集控制 样本，临床试验患者样本，流行病学数据，以及 临床结果数据。储存库将提供对血清的访问， 爱滋病患者的血浆和尿液。总RNA 制备，新鲜和石蜡包埋的淋巴瘤和KS组织样本 将与基线流行病学数据和临床数据一起提供 回应。

The UCLA and USC AIDS programs operate specimen repositories as resource laboratories for their respective research communities. Because of the unique resources of each of these repositories, significant extramural collaborations exist. These laboratories are designed to support clinical trials as well as basic science and epidemiologic investigations of the pathogenesis of AIDS related malignancies. Cedars Sinai Medical Center is a community based tertiary care hospital which acts as the referral center for AIDS related lymphoma pathology studies in Los Angeles. Together these three centers access over 200 new cases of Kaposi sarcoma and 100 cases of lymphoma each year. At UCLA, the laboratory' houses a computerized inventory of over 15,000 serum samples, 600 plasma samples and 800 frozen viable lymphocyte preparations. The bank provides isolates of Kaposi sarcoma cells and AIDS lymphoma cells for basic science studies. The samples are linked to clinical status and outcome as a result of participation in clinical trials. Most patients have multiple samples dating over 5 years providing longitudinal data. At USC, the laboratory houses over 5000 serum samples, 306 urine samples, 84 RNA/tissue preparations of Kaposi sarcoma lesions, and over 20 fresh Kaposi sarcoma cell lines. The majority of the samples were collected from an extensive epidemiologic survey designed to elucidate pathogens or processes leading to the development of Kaposi sarcoma or from clinical trials of anti-KS and anti-lymphoma agents. Collaborators have used these samples to exclude Rochelimea as a causative agent for KS, examine the relationship between cytokine perturbations and the development of lymphomas, identify key molecules in the post-receptor pathway of signal transduction in KS leading to proliferation, demonstrate that heavy chain utilization in lymphomas is non-random, investigate the clonality of AIDS malignancies, and isolated genetic fragments of a organism that maybe linked to classical and AIDS-related KS. At Cedars Sinai, over 235 cases of AIDS related lymphomas have been analyzed to date. These studies include extensive immunophenotypic characterization, EBV studies and delineation of p53 mutations. To date, these three repositories have serviced over 25 investigators at multiple institutions on a wide range of basic science questions requiring clinical materials. The unique ability of these banks to provide rare specimens (such a female or HIV negative epidemic KS cell lines) and the associated clinical information needed to place the results in context are the greatest strength of this repository. This application is for a joint repository to house samples related to AIDS related malignancies at UCLA after processing at the local sites. The application blends the individual strengths of each of the sample repositories with the talents of accomplished AIDS malignancies investigators. The bank already contains a wealth of samples vital to the study of Kaposi sarcoma and lymphoma pathogenesis. Valuable control samples from matched community based controls are unique to this repository. The combined repository will continue to collect control samples, samples from patients on clinical trials, epidemiologic data, and clinical outcome data. The repository will provide access to serum, plasma, and urine from patients with AIDS malignancies. Total RNA preparations, fresh and paraffin embedded lymphoma and KS tissue samples will be available along with the baseline epidemiologic data and clinical response.