CELLULAR AND MOLECULAR STUDIES OF RENAL TRANSPORT

寻求支持的上皮和 肾脏研究 将采用多种方法进行调查 电解质和流体穿过极化细胞的输送机制， 尤其是肾单位的那些。 各组成项目将调查 分子传输操作（离子通道、泵和耦合 运输）介导Na、K、C1和H运输，使用分离的小管， 肾和系膜细胞培养物，单个肾小管细胞，肾膜 和转运蛋白。 广泛的研究不仅包括 努力确定转运蛋白的分子结构， 了解它们的分布和插入机制 进入上皮细胞膜 离子转运蛋白与 他们的规定也将进行研究。 几个核心设施将支持强互动的研究 项目 这些核心单元包括用于开发和维护 电子设备、用于成像技术的扩展单元和细胞 文化核心设施。 通过提供关于分子和细胞的新信息， 离子和流体输运机制，我们的研究也将提高 认识和治疗肾脏疾病与临床电解质 紊乱 它们还可以为开发新的 副作用少的利尿剂。

Support is sought for a broad and integrated program in epithelial and renal research. A wide range of approaches will be used to investigate the mechanisms of electrolyte and fluid transport across polarized cells, especially those of the nephron. The component projects will investigate the molecular transport operations (ion channels, pumps and coupled transport) mediating Na,K, C1 and H transport, using isolated tubules, renal and mesangial cell cultures, single tubule cells, renal membranes and transport proteins. The broad range of studies will include not only effort to identify the molecular structure of transport proteins but also to understand the mechanisms governing their distribution and insertion into epithelial membrane. The interaction between ion transporters and their regulations will also be studies. Several core facilities will support the strongly interactive research projects. These core units include one for developing and maintaining electronic equipment, and expanded unit for imaging techniques and a cell culture core facility. By providing novel information about the molecular and cellular mechanisms of ion and fluid transport, our studies also will improve the understanding and treatment of renal diseases and clinical electrolyte disorders. They also may provide a rational basis for developing new diuretics with fewer harmful side effects.