ELECTRIC FIELDS ON METABOLISM OF A CHRONIC WOUND MODEL

在接受康复医学治疗的患者中，有 压疮和腿部溃疡。这些都有很高的发病率和死亡率 而且往往对常规治疗有抵抗力。的功效。 电疗在使用了几十年后仍未解决，而且有可能 仍然是伤口内的自然电力来源(即 “皮肤电池”)可能会促进愈合。 我们建议详细阐述成纤维细胞减少蛋白质的观察 如果暴露在与内生电场相当的电场中，就会合成。 为此，人真皮成纤维细胞在I型胶原真皮中 “等价”矩阵(DEM)暴露在离子流中。首字母 我们的DEM模型的结果是有希望的。他们建议增加 在20 mV/m、10 HZ的电场下增殖(P<0.01)。(最常 合成和增殖是相互作用的)。 采用相同的模型，我们建议对 DEM模型对这些电场的生化响应。我们计划 DNA、蛋白多糖、胶原、纤维连接蛋白和 胶原酶。确定最容易受到电气影响的工艺 影响，我们将调查对 内生范围。如果皮肤电池对伤口愈合很重要， 电池在1-10赫兹(即降至5-20m/m)时最灵敏。 创伤后早期，成纤维细胞经历纤维增生，并合成 蛋白多糖和纤维连接蛋白。后来，胶原蛋白的合成和 新血管生成高峰。我们将研究生物化学的反应 刺激后0-14天DEM中的成纤维细胞 生物合成转化在体外发生。如果他们这样做了，我们就会有 令人信服的证据表明，皮肤电池有助于调节 活着。最后，我们将研究早期的电效应 最终导致增殖和/或 综合。

Among the patients treated by Rehabilitation Medicine are those with decubitus and leg ulcers. These have significant morbidity, mortality and often are resistant to conventional treatment. The efficacy of electrotherapy is unsettled after decades of use and the possibility remains that a natural source of electricity within wounds(i.e., the "skin battery") may promote healing. We propose to elaborate the observation that fibroblasts decrease protein synthesis if exposed to electric fields comparable to endogenous fields. For this purpose, human dermal fibroblasts in type I collagen dermal "equivalent" matrices (DEM) are exposed to ionic currents. Initial results from our DEM model are promising. They suggests an increase in proliferation to electric fields of 20 mv/m, 10Hz.(p,01). (Most often synthesis and proliferation are reciprocal). Employing the same model, we propose a comprehensive study of the biochemical response of the DEM model to these electric fields. We plan radioisotope assays for DNA, proteoglycans, collagen, fibronectin and collagenase. Identifying the process most susceptible to electric effect, we will survey response to amplitudes and frequencies within the endogenous range. If the skin battery is important to wound healing, cells should be most sensitive at 1-10 Hz (i.e., down to 5-20 m/m). Early after wounding, fibroblasts undergo fibroplasia, and synthesis of proteoglycans and fibronectin. Later, collagen synthesis and neoangiogenisis peak. We will investigate the biochemical response of fibroblasts in DEM from 0 to 14 days after stimulation to see if these biosynthetic transformations occur in vitro. If they do, we will have compelling evidence that the skin battery help mediates the response in vivo. Lastly, we will investigate electric effects on early intracellular events that ultimately lead to proliferation and/or synthesis.