ROLE OF LIPIDS IN UROLITHIASIS

描述（改编自申请人的摘要）：在美国， 每1,000家医院中有7 - 10家患有肾结石 每1,000人中就有1人患有结石病 在他们的一生中。 大约80%的结石含有钙 草酸、尿酸和鸟粪石约占10- 15%。 公 接受，一个重要的事件，在石头开始是异质性 成核的石头晶体，和矩阵作为一个主要的 有机成核材料的来源。 特别值得注意的是 酸性磷脂存在于尿液中， 细胞损伤，受损细胞脱落进入尿液，细胞 死亡，以及膜成分释放到尿液中。 根据骨生长领域的理论， 磷灰石形式的磷酸钙经常被发现， 草酸钙结石的病灶，应用假设，酸性 来自肾小管质膜的磷脂形成复合物 与钙和无机磷酸盐结合，然后有序化并变成磷灰石。 首席研究员和他的同事们是唯一一个 研究组探讨结石病的这种可能性。 这是一个重要 这是一个问题，肯定需要进一步调查。 这项研究的主要目的是调查 草酸钙、鸟粪石和尿酸结石中的脂质， 单个脂质，并确定它们在钙成核中的作用 草酸盐晶体。 实验计划将比较脂质 在这些结石中确定的脂质组成与在 肾上皮细胞质膜。 这些研究的假设是， 草酸钙结石会含有磷脂，尿酸结石会 含有较少的磷脂含量，和鸟粪石（形成于 氨分解细菌存在）将含有两种复合的 糖脂以及磷脂。

DESCRIPTION (Adapted from the Applicant's Abstract): In the United States, renal stone disease accounts for 7 to 10 out of every 1,000 hospital admissions and 1 of 1,000 individuals are afflicted with stone disease during their lifetime. Approximately 80% of all stones contain calcium oxalate, and uric acid and struvite accounts for about 10-15%. It is well accepted that an important event in stone initiation is the heterogeneous nucleation of stone crystals, and that matrix serves as one of the major sources of organic nucleation materials. Of special interest are the acidic phospholipids which are present in the urine probably as the result of cellular injury, sloughing of the damaged cells into the urine, cell death, and the release of membrane components into the urine. Following the theories expressed in the bone growth field, and the fact that calcium phosphate in the form of apatite is frequently found as the nidus in calcium oxalate stones, the application hypothesizes that acidic phospholipids derived from the renal tubule plasma membrane form a complex with calcium and inorganic phosphate which then orders and becomes apatite. The principal investigator and his colleagues have been the only research group to explore this possibility in stone disease. This is an important subject and definitely requires further investigation. The main goal of the proposed research is to investigate the presence of lipids in calcium oxalate, struvite, and uric acid stones, identify the individual lipids and determine their role in the nucleation of calcium oxalate crystals in vitro. The experimental plan will compare the lipid composition determined in these stones with the lipid composition found in renal epithelial plasma membranes. The hypothesis in these studies is that calcium oxalate stones will contain phospholipids, uric acid stones will contain less phospholipid content, and struvite stones (which are formed in the presence of ammonia-splitting bacteria) will contain both complexed glycolipids as well as phospholipids.