CORNEAL EPITHELIAL CELL SURFACE GLYCOCONJUGATES

这项由两部分组成的研究将检查角膜感染的发病机制和 角膜上皮细胞迁移的机制。 (1)铜绿假单胞菌和金黄色葡萄球菌在角膜上的粘附性： 本研究旨在进一步阐明细菌附着的机制。 到角膜上皮细胞。不同类型兔角膜的化学结构 上皮细胞表面糖鞘糖脂(GSL)与P. 铜绿假单胞菌和金黄色葡萄球菌将被阐明。我们将尝试通过以下方式确定 组织化学技术采用抗GSL单抗，无论P。 兔角膜上皮中发现的铜绿假单胞菌和金黄色葡萄球菌反应性GSL 在正常人和兔角膜上皮细胞上存在培养。 活体，(Ii)遮盖，直到伤害导致它们暴露，或(Iii)存在 仅在受损的人和兔的角膜上皮细胞上。实验还将 以确定是否有可能(I)阻止粘连 不同抗GSL单抗对受损角膜细菌的杀伤作用 抗体、糖、寡糖、凝集素和糖苷酶以及(Ii) 用多种糖类洗脱损伤角膜中的粘附菌 半个。希望这项研究将有助于我们理解 细菌性角膜溃疡的发病机制。 (2)角膜上皮伤口愈合：本项目的目标是 进一步阐明角膜上皮细胞迁移的机制。这个 兔角膜移行特异性GSL的化学结构 上皮学将被阐明。我们将尝试通过组织化学方法确定 技术是否具有兔角膜移行特异性GSL。它还将 确定是否有可能刺激角膜上皮 在器官培养中添加纯化的角膜移行损伤的角膜 将特定于迁移的GSL迁移到培养介质。实验也将是 以确定蛋白水解酶是否在角膜上皮起作用 通过暴露遮盖的角膜上皮细胞表面GSL和 这些GSL通过与细胞相互作用影响细胞迁移 细胞外基质的大分子。希望这项研究 将有助于我们理解那些调节角膜的因素 上皮性伤口愈合。

This two-part study will examine the pathogenesis of corneal infection and mechanisms of corneal epithelial cell migration. (1) Adherence of P. aeruginosa and S. Aureus to the Cornea: The goals of this study are to further elucidate the mechanism of bacterial attachment to corneal epithelium. The chemical structure of various rabbit corneal epithelial cell surface glycosphingolipids (GSL) which bind to P. aeruginosa and S. aureus will be elucidated. We will try to determine by histochemical techniques using anti-GSL monoclonal antibodies whether P. aeruginosa nd S. aureus-reactive GSL found in rabbit corneal epithelium in culture are (i) present on normal human and rabbit corneal epithelium in vivo, (ii) masked until injury causes them to be exposed, or (iii) present only on injured human and rabbit corneal epithelium. Experiments will also be performed to determine whether it is possible (i) to block the adherence of bacteria to the injured cornea by various anti-GSL monoclonal antibodies, sugars, oligosaccharides, lectins, and glycosidases and (ii) to elute the adherent bacteria from the injured corneas by various saccharide moieties. It is hoped that this study will contribute to our understanding of the pathogenesis of bacterial corneal ulcers. (2) Corneal Epithelial Wound Healing: The goal of this project are to clarify further the mechanism of corneal epithelial cell migration. The chemical structure of the migration-specific GSL of rabbit corneal epithelium will be elucidated. We will try to establish by histochemical techniques whether migration-specific GSL of rabbit corneas. It will also be determined whether it is possible to stimulate corneal epithelial migration of wounded corneas in organ culture by the addition of purified migration-specific GSL to the culture media. Experiments will also be performed to establish whether proteases play a role in corneal epithelial cell migration by exposing the masked corneal epitheal cell surface GSL and that these GSL influence cell migration by interacting with the macromolecules of the extracellular matrix. It is hoped that this study will contribute to our understanding of those factors that modulate corneal epithelial wound healing.