CORE--CELL CULTURE

了解囊性纤维化病理生物学的基础研究 需要连续和可靠的细胞来源，从 患有和不患有这种疾病的患者。因此，该计划的主要目标是 核心呼吸道细胞培养设施是为了获得呼吸道组织和培养 呼吸道表面上皮细胞和气管支气管腺细胞。一张纸巾 用于获得正常和CF呼吸道组织的采购系统已经 已经建立了。一旦获得，组织就会被酶消化，然后 在原代培养中建立分散的细胞。气管、支气管镜 腺体细胞通过单个通道扩张。表面上皮细胞和 腺体细胞培养的分化特性的评估使用 光学显微镜、电子显微镜和短路测量 电流和跨上皮阻力。汇合的细胞薄片是 分发给调查人员。此外，已建立的细胞系 调查人员日常使用的数据由核心维护和分发 细胞培养设施。细胞培养核心也将继续其 努力改善文化，特别是那些源自 气管、支气管腺细胞。基质添加剂(生长)的效果 因素、激素、其他化学物质)对气道腺细胞生长和 将进行差异化测试。空中界面投放的效果和效果 多种生长载体(例如，不同的滤膜、各种胶原蛋白 和细胞外基质成分)也将得到评估。这些 实验应该允许我们确定一组相对简单的 能充分表达离子转运和粘液的培养条件 来自低电镀密度的分泌功能。

Basic research toward understanding the pathobiology of cystic fibrosis requires a continuing and dependable source of cells, obtained from patients with and without the disease. Thus, the primary objective of the CORE Airway Cell Culture Facility is to acquire airway tissues and culture airway surface epithelial and tracheobronchial gland cells. A tissue procurement system for obtaining normal and CF airway tissue has already been established. Once obtained, tissue is enzymatically digested and the dispersed cells are established in primary culture. Tracheobronchial gland cells are expanded through a single passage. Surface epithelial and gland cell cultures are evaluated for differentiated properties using light microscopy, electron microscopy and measurements of short circuit current and transepithelial resistance. Confluent cell sheets are distributed to investigators. Additionally, established cell lines routinely used by investigators are maintained and distributed by the CORE Cell Culture Facility. The Cell Culture CORE will also continue its efforts to improve cultures, particularly those derived from tracheobronchial gland cells. The effects of media additives (growth factors, hormones, other chemicals) on airway gland cell growth and differentiation will be tested. The effects of air interface feeding and of a variety of growth supports (e.g. different filters, various collagens and extracellular matrix components) will also be assessed. These experiments should allow us to determine a relatively simple set of culture conditions which allows full expression of ion transport and mucus secretory function from low plating densities.