MICROBIAL METABOLISM ASSAY SYSTEM

描述：(改编自申请人的摘要)。我们的目标是 一种新型微生物体外检测光学系统的研制 新陈代谢。它提供了独特的机会来分析 有没有活的生物体，监测微生物的活动，以及 对微生物进行毒性或刺激性条件测试。这个 方法测量样品中的耗氧量。它是实用的 对多个小样本进行快速、经济的分析。 该方法创新性地使用氧气敏感指示剂进行检测。 样本。指示剂的光谱性质在氧气中发生变化- 浓度依赖方式。样品的含氧量为 通过使用外部设备询问容器中的指示器来确定 光学阅读器。第二阶段的目标是提高敏感性 改进了检测系统的传感设计，使其适应于 技术应用于小样本的检测。调查将会是 以确定其在生物检测和无菌检测中的价值 与传统技术相比，样品的采集时间更短。 该系统将是通用的和有用的许多应用程序范围 从无菌测试、毒性测试和微生物研究，到 治疗性药物开发。它有广泛的潜力用于 涉及微生物的检测，或在诊断中进行微生物检测， 研究、环境和许多商业应用。

DESCRIPTION: (Adapted from the Applicant's Abstract). The objective is to develop a novel optical system for in vitro assay of microbial metabolism. It uniquely presents opportunities to assay for the presence or absence of viable organisms, monitor microbial activity, and conduct tests with microbes for toxic or stimulatory conditions. The method measures oxygen consumption in samples. It is practical for conducting rapid, economical analyses of multiple small samples. The approach innovatively uses oxygen sensitive indicators in assay samples. Spectral properties of the indicator change in an oxygen- concentration dependent manner. The oxygen content of samples is determined by interrogation of indicators in vessels using an external optical reader. The aims of Phase II are to increase the sensitivity of the assay system, improve the sensing design, and adapt the technology to the assay of small samples. Investigations will be conducted to establsih its value in bioassays and testing sterility of samples in less time than conventional techniques. The system will be versatile and useful for numerous applications ranging from sterility tests, toxicity testing, and microbial research, to therapeutic drug development. It has widespread potential for use in assays that involve microbes, or testing for microbes in diagnostic, research, environmental and many commercial applications.