PHYSIOLOGY AND PHARMACOLOGY OF CARDIOVASCULAR NEURONS

电生理和解剖技术的最新进展 提供了一个机会来检查中枢神经成分， 参与调节神经流出到心脏的反射回路， 血管 这里提出的工作的第一部分是一个 继续进行电生理学和免疫细胞化学研究 着重于孤束核内侧核的髓细胞 束（mNTS），参与整合来自 动脉压力感受器和其他外周和中枢源。 的 研究集中在mNTS神经元，这些神经元直接受 压力感受器传入纤维 第二部分的工作开始一个 详细检查压力感受器神经元的特性（ 突触前神经元），其将动脉压信息传递到 mNTS（postsynaptic neurons） 最近的进展使 这两组细胞的解剖学鉴定， 以及接受主动脉压力感受器神经支配的那些。 大鼠和 以豚鼠为动物模型。 第一个目标是评估 六个电压依赖性离子电流的作用， 接受压力感受器输入的mNTS神经元的自发活动 并确定该细胞传递动作电位的能力 压力感受器反射通路中的下一个神经元。 这项研究，利用 用于电压和/或电流记录的制霉菌素移液管技术建立在 在上一个授予期间获得的数据。 第二个目标继续 对负责兴奋性和抑制性的递质的研究 调节mNTS活性。 为了补充我们以前的研究， 谷氨酸和GABA，我们有针对性的几个潜在的发射器的基础上， 在我们的免疫细胞化学研究中， 左主动脉减压神经 第三个目标将继续执行这项建议 压力感受器释放递质的突触前调制 纤维的发生是对儿茶酚胺、AII、生长抑素、CGRP P物质和/或GABA。 这些发射器对 将探索主动脉压力感受器神经元的电活动。

Recent developments in electrophysiological and anatomical techniques have provided the opportunity to examine the central neural components of the reflex loops involved in regulating neural outflow to the heart and blood vessels. The first part of the work proposed here is a continuation of an electrophysiological and immunocytochemical study focusing on the medullary cells in the medial nucleus of the solitary tract (mNTS) that are involved in integrating information from the arterial baroreceptors and other peripheral and central sources. The studies focus on the mNTS neurons that are directly innervated by baroreceptor afferent fibers. The second part of the work begins a detailed examination of the properties of the baroreceptor neurons (the presynaptic neurons) that carry the arterial pressure information to the mNTS (the postsynaptic neurons). Recent progress has permitted anatomical identification of both groups of cells, those that transmit and those that receive the aortic baroreceptor innervation. Rats and guinea pigs are used as the animal models. The first aim is to assess the role of the six voltage dependent ionic currents that underlie spontaneous activity in the mNTS neuron that receives baroreceptor input and to determine the ability of this cell to transmit action potentials to the next neurons in the baroreflex pathway. This study, utilizing the nystatin pipette technique for voltage and/or current recording builds on data obtained in the previous granting period. The second aim continues a study of the transmitters responsible for excitatory and inhibitory modulation of mNTS activity. To complement our previous studies on glutamate and GABA we have targeted several potential transmitters based on our immunocytochemical studies of the region of the mNTS innervated by the left aortic depressor nerve. The third aim will pursue the proposal that presynaptic modulation of transmitter release from baroreceptor fibers occurs in response to the catecholamines, AII, somatostatin, CGRP substance P, and/or GABA. The action of these transmitters on the electrical activity of the aortic baroreceptor neurons will be explored.