IN SITU RAMAN SPECTROSCOPIC ANALYSIS OF HUMAN ARTERIAL D

这项建议的长远目标是发展近红外（NIR） 拉曼光谱作为一种定量方法用于原位检测 人外周动脉粥样硬化及其进展。与 这项技术，化学成分和分子身份的信息 可以快速、准确、远程地获得。因此， 提供定量测量组织化学和 组织病理学标志物的存在和程度 动脉粥样硬化改变 我们建议的具体目标是：l）调查和验证 近红外拉曼光谱在诊断和预后中的应用 肌肉和弹性动脉的动脉粥样硬化。我们提出了几 充分表征光谱可变性的方法， MR拉曼信号与疾病相关的动脉改变， 建立对不同生化部分的检测灵敏度 和代谢物。我们已经计划了具体的实验来比较拉曼和 化学分析，将在不同温度下观察到的光谱特征 动脉粥样硬化的阶段与形态学和分子变化 并使用这些信息来建立诊断决策 阴谋2)发展振动光谱技术用于临床 应用.详细的实验概述，以提高近红外拉曼 用于临床应用的系统，并建立新的光谱系统， 包括用于手术室的便携式系统和拉曼 微探针3)确认光谱模型和诊断的有效性 临床前研究中的算法。对组织的体内研究 动脉粥样硬化引起的改变的目的是 确定体外开发技术的可转移性。 这项调查所产生的技术将广泛适用 各种人类动脉疾病的特征， 广泛地说，其他组织系统，并将建立真实的能力， 人体组织组织化学的体内时间测定， 病理生物学

The long range goal of this proposal is to develop near infrared (NIR) Raman spectroscopy as a quantitative method for in situ detection of atherosclerosis and its progression in human peripheral arteries. With this technique, information on chemical composition and molecular identity can be obtained rapidly, accurately, and remotely. Therefore, it has the potential to provide quantitative measurement of histochemical and histopathological markers for both the presence and extent of atherosclerotic alterations. The specific goals of our proposal are to: l) Investigate and validate the use of NIR Raman spectroscopy for diagnosis and prognosis of atherosclerosis in muscular and elastic arteries. We propose several approaches for characterizing fully the spectroscopic variability of the MR Raman signals with disease-associated alterations of artery, and establishing the detection sensitivity to different biochemical moieties and metabolites. We have planed specific experiments to compare Raman and chemical assays, correlate the spectral features observed at different stages of atherosclerosis with the morphological and molecular changes that take place, and use this information to establish diagnostic decision schemes. 2) Develop vibrational spectroscopy techniques for clinical applications. Detailed experiments are outlined to improve NIR Raman systems for clinical applications and build new spectroscopic systems, including a portable system for use in an operating room and Raman microprobes. 3) Confirm the validity of spectral models and diagnostic algorithms in preclinical studies. An in vivo study of the tissue alterations caused by atherosclerosis is proposed with the objective of determining the transferability of the technologies developed in vitro. The technique generated by this investigation will be broadly applicable to the characterization of disease in various human arteries and, more broadly, other tissue systems, and will establish the capability for real- time in vivo determination of human tissue histochemistry and pathobiology.