MICROBIAL METABOLISM ASSAY SYSTEM

描述：（改编自申请人摘要）。目标是 开发一种用于微生物体外检测的新型光学系统， 新陈代谢.它提供了独特的机会， 是否存在活微生物，监测微生物活性，以及 用微生物进行毒性或刺激性条件的测试。的 方法测量样品中的耗氧量。是实用 对多个小样本进行快速、经济的分析。 该方法创新性地使用氧敏指示剂进行分析 样品氧气中指示剂的光谱特性变化- 浓度依赖方式。样品的氧含量为 通过使用外部传感器询问容器中的指示器来确定 光学阅读器第二阶段的目标是提高灵敏度， 分析系统，改进传感设计，并适应 技术用于小样品的分析。调查将 进行了生物测定和无菌测试，以确定其价值 在更短的时间内比传统技术的样品。 该系统将是多功能和有用的许多应用， 从无菌测试、毒性测试和微生物研究， 治疗药物开发。它具有广泛的应用潜力， 涉及微生物的测定，或在诊断中检测微生物， 研究、环境和许多商业应用。

DESCRIPTION: (Adapted from the Applicant's Abstract). The objective is to develop a novel optical system for in vitro assay of microbial metabolism. It uniquely presents opportunities to assay for the presence or absence of viable organisms, monitor microbial activity, and conduct tests with microbes for toxic or stimulatory conditions. The method measures oxygen consumption in samples. It is practical for conducting rapid, economical analyses of multiple small samples. The approach innovatively uses oxygen sensitive indicators in assay samples. Spectral properties of the indicator change in an oxygen- concentration dependent manner. The oxygen content of samples is determined by interrogation of indicators in vessels using an external optical reader. The aims of Phase II are to increase the sensitivity of the assay system, improve the sensing design, and adapt the technology to the assay of small samples. Investigations will be conducted to establsih its value in bioassays and testing sterility of samples in less time than conventional techniques. The system will be versatile and useful for numerous applications ranging from sterility tests, toxicity testing, and microbial research, to therapeutic drug development. It has widespread potential for use in assays that involve microbes, or testing for microbes in diagnostic, research, environmental and many commercial applications.