NEW APPROACHES TO FLOW SORTING OF CHROMOSOMES

通过流动分选来纯化单个染色体通常是第一次 定义正常和异常人类基因结构的步骤。脱氧核糖核酸 扩增技术大大减少了分选的数量 所需材料，使开发更小、成本更低的产品成为可能 分拣员。使用单束空气的双光束仪器的性能- 制冷氦-镉(He-Cd)激光器作为紫外光源 和蓝色激发光用于流式细胞术染色的染色体 DAPI/奥立夫霉素或Hoechst 33258/色霉素A3将评估为 确定该系统是否可以解析人类染色体组型以及 可以将现有的商业设备。此外，几个A-T和G-C 偏爱以前未应用于染色体分析的DNA荧光染料 并且有可能在大量现有的流分类器中使用 单光束488或515 nm激发将作为替代方案进行检查 目前用于双变量Flow核型分析的染色。

Purification of individual chromosomes by flow sorting is often a first step in defining the structure of normal and abnormal human genes. DNA amplification techniques have greatly reduced the amount of sorted material required, making it feasible to develop smaller, lower-cost sorters. The performance of a dual-beam instrument using a single air- cooled helium-cadmium (He-Cd) laser as a source of both ultraviolet (UV) and blue excitation light for flow Cytometry of chromosomes stained with DAPI/olivomycin or Hoechst 33258/chromomycin A3 will be evaluated to determine whether the system can resolve the human karyotype as well as can existing commercial apparatus. In addition, several A-T and G-C preferring DNA fluorochromes not previously applied to chromosome analysis and potentially usable in a large number of existing flow sorters with single-beam 488 or 515 nm excitation will be examined as alternatives to stains presently used for bivariate flow karyotyping.