BIOANALYTICAL APPLICATIONS OF ACOUSTO-OPTIC DEVICES

描述（改编自申请人摘要）：本研究的目的 建议是开发新的测量仪器不可能 使用现有的技术。声光的独特性质 可调谐滤波器（AOTF）将被开发。具体来说， 的AOTF给出衍射光在一个以上的波长， 以不同频率调制的每个波长将 与热透镜技术协同结合， 毛细管多波长红外热透镜检测器 电泳（CE）几乎为零背景，高灵敏度， 和普遍性。此外，该探测器将能够 通过IR发色团识别样品中的官能团。 该检测器可特别用于大分子的测序 例如DNA。一种灵敏、通用的高效液相色谱和毛细管电泳手性检测器 将通过将热透镜效应与AOTF相结合来开发， 测量振动圆二色性。用于紫外和可见光的AOTF 区域将用于随后的快速扫描构造 吸收和圆二色性（CD）检测器用于HPLC。 优势 最近开发的集成AOTF将被用于 对仪器进行数字化处理，并为其提供附加功能 例如更快的扫描能力和消除重新对准。最后， AOTF能够反射来自不同位置的光， 一个具有不同波长的物体将被用来制造一个 凝胶和毛细管阵列电泳（GE和CAE）检测器， 具有真正的超多光谱成像能力（即，它能够 以空间分辨率记录对象的每个点的光谱， 高到与光谱分辨率相当）。因为这 检测器具有高灵敏度和比其它检测器更高的通量， 它可能为DNA测序提供重大突破。总的来说， 所获得的结果将为科学家提供必要的工具， 可以容易地用于灵敏和快速地检测，并且（化学和 立体化学地）测定化学和生物化学样品（例如， 致癌化合物、药物、DNA、蛋白质），因为它们被 HPLC、CE和GE。这些工具对人类的贡献 基因组计划将是无价的，因为他们将提供大量的 GE和CAE技术的产量增加。

DESCRIPTION (Adapted from applicant's abstract): The objective of this proposal is to develop novel instruments for measurements not possible using existing techniques. The unique properties of acousto-optic tunable filters (AOTFs) are to be exploited. Specifically, the ability of the AOTF to give diffracted light at more than one wavelength with each wavelength modulated at a different frequency will be synergistically combined with the thermal lens technique to make a multiwavelength infrared thermal lens detector for capillary electrophoresis (CE) with virtually zero background, high sensitivity, and universality. Additionally, the detector will be capable of identifying functional groups in a sample through their IR chromophores. The detector may be particularly useful for sequencing of macromolecules such as DNA. A sensitive and universal chiral detector for HPLC and CE will be developed by combining the thermal lens effect with the AOTF to measure vibrational circular dichroism. An AOTF for the UV and visible regions will be made for subsequent construction of a rapid scanning absorption and circular dichroism (CD) detector for HPLC. Advantages of the recently developed integrated-AOTF will then be taken to miniaturize the instruments and to provide them with additional features such as faster scan capability and elimination of realignment. Finally, the AOTF's ability to diffract light coming from different positions of an object with different wavelengths will be exploited to make a detector for gel and capillary array electrophoresis (GE and CAE) that has true supermultispectral imaging capability (i.e., it is capable of recording spectra for each point of an object with spatial resolution so high that it is comparable to the spectral resolution). Because this detector has high sensitivity and higher throughput the other detectors, it may provide a major breakthrough for DNA sequencing. Collectively, results obtained will provide the necessary instruments that scientists can easily use to detect sensitively and rapidly and (chemically and stereochemically) to determine chemical and biochemical samples (e.g., carcinogenic compounds, drugs, DNA, proteins) as they are separated by HPLC, CE, and GE. The contribution of these instruments to the human genome project will be invaluable because they will provide substantial increases in the throughputs of the GE and CAE techniques.