MBRS

这个项目建议研究低分子结构和高分子结构。 查尔西青霉菌丝体糖肽的重量及含量测定 无论这些聚合物是作为前体和/或连接点还是 肽磷半乳甘露聚糖(PPGMS)或胞外的锚定 与之共同释放的糖水解酶、磷酸酶和蛋白酶 PPGMS。为了建立前体/产品之间的关系 菌丝体糖肽和胞外pPGMS的结构 我们将对细胞壁、细胞膜和胞外PPGMS进行表征。 糖肽之间的结构关系将是 一维和二维核磁共振波谱和GC/MS研究 多肽的光谱分析与氨基酸分析 组件。此外，生物合成关系将通过以下方式进行探索 使用放射性标记的碳水化合物和多肽前体并通过 细胞壁的生物素化，然后分离出相应的 来自细胞壁、膜和胞外部分的糖肽。 建立这些糖肽在细胞壁上的锚定或 膜对于理解生理事件很重要。 导致PPGMS、水解酶和潜力的释放 来自查尔西青霉的过敏原。

This project proposes to examine the structures of low and high molecular weight mycelial glycopeptides from Penicillium charlesii and to determine whether these polymers serve as precursors and/or points of attachment or anchors for peptidophosphoglactomannans(pPGMs) or the extracellular glycohydrolases, phosphatases and proteases that are co-released with pPGMs. In order to establish the precursor/product relationships between the mycelial glycopeptides and the extracellular pPGMS, the structures of the cell wall, membrane, and extracellular pPGMs will be characterized. The structural relationships between the glycopeptides will be investigated by one and two dimensional NMR spectroscopy and GC/mass spectrometry in conjunction with amino acid analyses of peptide components. In addition, the biosynthetic relationships will be probed by using radiolabeled carbohydrate and peptide precursors and by biotinylation of cell walls, followed by isolation of the corresponding glycopeptides from the cell wall, membrane, and extracellular fractions. Establishing the anchor of these glycopeptides to the cell wall or membrane is important to the understanding of the physiological events leading to the release of pPGMs, hydrolytic enzymes, and potential allergens from the Penicillium charlesii.