TRANSFERRED RESISTANCE TO SCHISTOSOMES IN SNAILS

光滑双脐螺天然抗性机制的研究 蜗牛感染曼氏血吸虫的情况尚不清楚， 特别是造血组织的作用，所谓的 变形细胞产生器官（APO），在这种阻力是不清楚的。 一 最近的演示表明， 从人的APO移植物中获得的过继性耐药转移 遗传抗性捐赠者 本项目旨在阐明 这种转移阻力的机制，即，无论是由于 产生血细胞（血细胞嵌合）和/或分泌可溶性 植入物的“阻力因素”，并确定是否有额外的 免疫活性组织存在于蜗牛中。 APO同种异体移植受者 将检查暴露于以下物质后有丝分裂活性是否增加： 毛蚴增多，循环血细胞增多， 存在供体血细胞DNA，以及存在可溶性因子 通常仅在抗性血浆中发现，即，酵母调理和 孢子囊凝集活性。 其他组织和器官 还将研究耐药蜗牛的转移能力， 可怕的抵抗。 最后，几个可能影响 将检查转移的耐药性：供体的年龄， 植入物和植入物的激活状态。 的潜在 这项研究的意义在于，它可以帮助确定特定的 与对染色体的先天抗性有关的器官、组织和细胞 感染 此外，如果证实存在血细胞嵌合体， APO的造血功能将得到验证。

The mechanisms underlying innate resistance of Biomphalaria glabrata snails to infection with Schistosoma mansoni are not known, and specifically the role of the hematopoietic tissue, the so-called amoebocyte-producing organ (APO), in such resistance is unclear. A possible approach to this problem is suggested by the recent demonstration of adoptive transfer of resistance with allografts of the APO from genetically resistant donors. This project seeks to elucidate the mechanism for such transferred resistance, i.e., whether it is due to production of hemocytes (hemocytic chimerism) and/or secretion of soluble "resistance factors" by the implant, and to determine whether additional immunocompetent tissues are present in snails. APO allograft recipients will be examined for increased mitotic activity following exposure to schistosome miracidia, increased numbers of circulating hemocytes, presence of donor hemocyte DNA, and the presence of soluble factors normally found only in resistant plasma, i.e., yeast-opsonizing and sporocyst-agglutinating activity. Additional tissues and organs of resistant snails will also be studied for their ability to transfer schistosome resistance. Finally, several variables that may affect transferred resistance will be examined: age of the donor, size of the implant, and state of activation of the implant. The potential significance of this research is that it may help identify specific organs, tissues, and cells involved in innate resistance to schistosome infection. Furthermore, should hemocytic chimerism be demonstrated, the hematopoietic function of the APO will have been verified.