SOLID STATE DETECTOR FOR CAPILLARY ELECTROPHORESIS

电泳技术的最新进展开辟了 开发新型高性能仪器的可能性 在生物学研究中。 其中一项技术，毛细管电泳 (CE)，提供比传统凝胶更高的分辨率， 比传统的分离速度快一个数量级 技术. 也许它最大的优点是， 适合于结合在线检测系统， 自动化仪器的发展。 承包商的SBIR第一阶段计划解决了以下特殊需求： CE用于DNA的在线放射性检测系统 research. 第一阶段建造的原型探测器是由一个 小型碲化镉（CdTe）固态核辐射传感器， 发现提供83%的32 p和空间的检测效率 分辨率为1.25 mm。因此，这些新传感器非常适合 放射性标记DNA的在线检测。 在第二阶段中，承包商应使用多元素阵列， 检测器在一个新的CE仪器的发展-一个自动化的 限制性片段分析仪。 这种方法将使之有可能 利用CE的高分辨率和速度，在线检测 放射性DNA片段，以帮助构建DNA限制 网站地图

The recent advances in electrophoresis technology have opened up the possibility for the development of new, high performance instrumentation in biologicl research. One such technology, capillary electrophoresis (CE), provides much greater resolution than traditional gels and run speeds as much as an order of magnitude faster traditional separation techniques. Perhaps its greatest virtue is that the technique is quite amenable to the incorporation of on-line detection systems and the development of automated instrumentation. The Contractor's SBIR Phase I program addressed the particular need for an on-line radioactivity detector system which allow CE to be used in DNA research. The prototype detector built in Phase I was constructed from a small cadmium telluride (CdTe) solid-state nuclear radiation sensor and was found to provide 83% detection efficiency for 32p and a spatial resolution of 1.25 mm. Therefore, these new sensors are well-suited for on-line detection of radiolabeled DNA. In Phase II, the Contractor shall use multielement arrays of these detectors in the development of a new CE instrument - an automated restriction fragment analyzer. This approach will make it possible to utilize the high resolution and speed of CE with on-line detection of radioactive DNA fragments to aid in the construction of DNA restriction site maps.