BIOMEDICAL SEPARATIONS--FIELD FLOW FRACTIONATION

描述：拟议工作的主要目标是开发 场流分离（FFF）及其各种子技术， 越来越强大的工具，用于分离和分析复杂的 生物医药胶体及高分子材料 起源理论和实验研究相结合， FFF的独特能力，以压裂和表征高 与健康科学相关的分子量材料。拟议 工作将分为几个类别。在第一类中， 研究人员将追求FFF的优化以产生更高的速度， 分辨率和便利性。他将最优化理论转化为 简单的实验室指南。他还将研究基础胶体 与FFF相关的现象，并继续开发新技术， 修改FFF。在第二类中，调查人员将 集中开发应用最广泛的FFF流FFF 技术.他将为高- 加速流FFF分析并进行其他研究以改进和 自动化这项技术。在第三类中，调查人员将 利用他的在线样品浓缩技术， 将沉降FFF与流动FFF耦合，用于表征 复杂的胶体流体，如细胞裂解物。第四类， 他将开发应用程序和新的应用策略。这些将 补充他的基础研究。申请工作将针对 生物细胞，运动细菌，蛋白质复合物，蛋白质包涵体 体、血浆中的胶体、脂质体和其他类别的 生物和生物医学材料。

DESCRIPTION: The main objective of the proposed work is to develop field-flow fractionation (FFF) and its various sub-techniques into increasingly powerful tools for the separation and analysis of complex colloidal and macromolecular materials of biological and pharmaceutical origin. Theoretical and experimental studies will be combined to exploit the unique capabilities of FFF to fractionate and characterize high molecular weight materials relevant to the health sciences. The proposed work will be divided into several categories. In the first category, the investigator will pursue the optimization of FFF to yield higher speed, resolution, and convenience. He will convert optimization theory to simple laboratory guidelines. He will also study fundamental colloidal phenomena related to FFF and continue to develop new techniques and modifications of FFF. In the second category, the investigator will intensively develop flow FFF, the most widely applicable of FFF techniques. He will characterize and develop ultrathin channels for high- speed flow FFF analysis and undertake other studies to improve and automate this technique. In the third category, the investigator will utilize his on- line sample concentration technique to implement the coupling of sedimentation FFF to flow FFF for the characterization of complex colloidal fluids such as cell lysates. In the fourth category, he will develop applications and new application strategies. These will complement his basic studies. Applications work will be directed at biological cells, motile bacteria, protein complexes, protein inclusion bodies, colloids in blood plasma, liposomes, and other classes of biological and biomedical materials.