LARGE-SCALE PRODUCTION & PURIFICATION OF COMPOUNDS WITH BIOLOGICAL ACTIVITY

生物技术股负责生产和生产 生物材料的提纯。该股承担着广泛的任务 的生物生产任务，如大规模的增长 原核细胞和真核细胞，生产各种 微生物和真核细胞中的化合物及其纯化 指生物化合物，尤其是蛋白质。这些产品，不是 可从商业来源获得，用于临床和 结构研究。 生物技术单位有能力处理各种生产 与广泛不同的主题相关的问题，通过实现 生理和技术方法。高效的流程开发 生产是可能的，因为该单位拥有两个一体化的 要素：完全可操作的多用途试生产 进行研究和加工的设施和能力 发展。 在过去的一年里，该股执行了大约200个不同的 大规模制剂：(1)培养各种微生物，如 重组大肠杆菌，重组酵母，白喉棒状杆菌， 霍乱弧菌和几种志贺氏菌；(2)繁殖大 各种哺乳动物细胞的体积；(3)提纯克量 几种蛋白质，如CRM(交叉反应物质)白喉 毒素和牛S抗原。 该股专注于开发更有效的方法，以 蛋白质回收。在这一领域进行了研究，以使用 膨胀床技术在胞内和胞内的同时回收 胞外蛋白。一种新的锚固繁殖方法 依赖哺乳动物细胞来产生分泌蛋白 还介绍了。

The Biotechnology Unit is responsible for the production and purification of biological material. The Unit undertakes a wide array of biological production tasks such as large-scale growth of procaryotic cells and eucaryotic cells, production of various compounds from microorganisms and eucaryotic cells, and purification of biological compounds especially proteins. These products, not available from commercial sources, are needed for clinical and structural studies. The Biotechnology Unit is capable of handling various production problems associated with widely different topics by implementing both physiological and technical approaches. Efficient process development and production is possible since the Unit possesses two integrated elements: a fully operational multi- purpose pilot production facility and the capability of conducting research and process development. During the last year the Unit performed approximately 200 different large-scale preparations: (1) growing various microorganisms such as recombinant E. coli, recombinant yeast, Corynebacteria diphtheria, Vibrio cholera, and several types of Shigella; (2) propagating large volumes of various mammalian cells; and (3) purifying gram quantities of several proteins such as CRM (cross-reacting material) diphtheria toxin and bovine S antigen. The Unit concentrates on the development of more efficient methods for protein recovery. In this area research was conducted for the use of expanded bed technique for the recovery of both intracellular and extracellular proteins. A new method for propagating of anchorage dependent mammalian cells for the production of secreted proteins was also introduced.