MU OPIATE RECEPTOR--CELLULAR AND SUBCELLULAR LOCALIZATIONS

Mu阿片受体(Mor)已被确认为主要的大脑。 受体位置与止痛剂和快乐性最相关 可卡因。细胞和亚细胞定位数据对于 有助于阐明这一重要大脑的功能特性 分子。在本财年，研究了MOR蛋白在人体内的分布。 老鼠的大脑有了很大的进步。光学显微镜揭示了细节 大鼠脑内MOR分布的分布 支配腹侧被盖区的纤维分布显著， 核周膜、纤维和神经末梢呈密集免疫反应。 伏隔核。对MOR的超微结构研究表明， 免疫反应主要分布在胞内和质膜。 与传统突触不易关联的局部化 脊髓背角和伏隔核的专门化。一些人 MOR定位于脑啡肽免疫阳性神经元的突触后， 部分脑啡肽免疫阳性成分含有明显的吗啡 自身受体。在炎症性和其他方面也寻求MOR监管 模特们。这些研究大大增加了关于 阿片类镇痛剂的这些重要部位的细胞定位 奖励，并强调这些受体介导的可能性 阿片类药物和阿片肽在不同部位的主要活性 来自突触的专门化。

The mu opiate receptor (MOR) has been identified as the principal brain receptor site best correlated with the analgesic and euphoric properties of cocaine. Cellular and subcellular localization data are important to help to elucidate the functional properties of this important brain molecule. In this FY, studies of the distribution of the MOR protein in rat brain were substantially advanced. Light-microscopy revealed details of the distribution of MOR distribution in rat brain that included remarkable fiber distributions innervating the ventral tegmental area, and densely-immunoreactive perikarya, fibers and nerve terminals in the nucleus accumbens. Ultrastructural studies of the MOR revealed that most immunoreactivity was found in intracellular and plasma membrane localizations not readily associated with conventional synaptic specializations in spinal cord dorsal horn and in nucleus accumbens. Some MOR sites were postsynaptic to enkephalin-immunopositive neural elements, and some enkephalin-immunopositive elements contained apparent MOR Aautoreceptors. MOR regulation was also sought in inflammatory and other models. These studies substantially add to information on the detailed cellular localizations of these important sites for opiate analgesia and reward, and underscore the possibility that these receptors mediate primarily activities of opiate drugs and opioid peptides at sites away from synaptic specializations.