OPIOIDS AND ORGAN PRESERVATION

这个项目研究了生理效应和潜在的 δ阿片肽作用的生化机制和 器官中相关蛋白因子HIT（冬眠诱导触发因子） 保存和保护。 在肺移植实验中， 用HIT保存24小时后，肺功能正常 当移植到宿主动物体内时。 在另一个实验中， 阿片肽DADLE检测潜在的心肌保护作用 使用离体兔心进行实验。 在将心脏置于 缺血时，心脏在一段时间内接受DADLE单次灌注 15分钟 DADLE预灌注的这一过程显著地 在18小时的全面治疗后改善心肌功能恢复 与对照组相比，4EC时的缺血。 我们目前正在检查 DADLE在某些类型的CNS损伤中的潜在用途。

This project examines the physiological effects and the underlying biochemical mechanisms of the action of the delta opioid peptides and a related protein factor HIT (Hibernation Induction Trigger) in organ preservation and protection. In a lung transplantation experiment, the lung, after 24 hours of preservation with the HIT, functioned normally when transplanted into the host animal. In another experiment, the delta opioid peptide DADLE was examined for potential myocardial protective action using isolated rabbit hearts. Before subjecting the heart to ischemia, the hearts received a single perfusion of DADLE over a period of 15 minutes. This procedure of DADLE preperfusion dramatically improved recovery of myocardial functions following 18 hours of global ischemia at 4EC when compared to controls. We are currently examining the potential use of DADLE in certain types of CNS damage.