BIOLOGICAL EFFECTS OF JOINT REPLACEMENT

该项目的目的是确定是否 接受全髋关节或膝关节置换术的患者， 含铬的医疗器械的 DNA-蛋白质交联（DPC）水平，一种高度敏感的标志物 早期的生物学损伤。具体 目标是： * 目的：探讨糖尿病患者外周血淋巴细胞DPC水平是否与糖尿病的发病有关。 关节置换手术后患者增加。 * 为了评估循环铬浓度的变化是否 与DPC水平的变化相关。 * 为了研究手术和水平升高之间的时间差， 的DPC。 由于髋关节置换患者已经被观察到在 增加结缔组织肿瘤的发展风险，以及 对于肾癌和前列腺癌，DPC测定具有 有可能成为一种监测工具， 含有铬的金属假体装置的后果。 将在接受以下治疗的患者中招募50名研究参与者： 髋关节或膝关节置换手术。他会事先采集血液 进行手术以建立基线测量。每个参与者 将在12个月内定期随访4次。在 除了DPC测定外，循环铬浓度还将 被衡量。

The purpose of the proposed project is to determine whether individuals undergoing total hip or knee replacement procedures with medical devices containing chromium experience an elevation in the levels of DNA-protein crosslinks (DPC), a highly sensitive marker of early biological damige from exposure to chromium. The specific aims are: * To assess whether the levels of DPC in peripheral lymphocytes of joint replacement patients increase after the surgical procedure. * To assess whether changes in circulating chromium concentration correlates with changes in levels of DPC. * To study the time-lag between surgery and the increase in levels of DPC. Since hip replacement patients have already been observed to be at increased risk for development of connective tissue tumors, as well as cancers of the kidney and prostate, the DPC assay has the potential to become a monitoring tool to detect any long-term health consequences from metal prosthetic devices containing chromium. Fifty study participants will be recruited among patients undergoing hip or knee replacement procedures. Blood will he collected prior to surgery to establish a baseline measurement. Each participant will be followed up at four periodic intervals for 12 months. In addition to the DPC assay, circulating chromium concentration will be measured.