REGENERATION OF CD4 LYMPHOCYTES

描述(改编自申请人的摘要)：本报告的主要目的 项目是确定(I)循环池的凋亡，还是(Ii)细胞 周期停滞是导致CD4细胞无法再生的原因之一，并且 (3)确定与之相关的潜在细胞损害。朝向 为了达到这个目标，调查人员将重点放在获得的纵向样本上 在抗病毒治疗的急性期。他们将由FACS决定 分析细胞因子受体的存在或强度、黏附 分子、凋亡标记物、DNA含量和限制点相关 细胞周期蛋白对表达Ki67的CD_4细胞及其体外细胞因子的影响 通过比较在显示不同的时间点获得的数据 CD4淋巴细胞再生的动力学，他们希望能更好地定义 增强或抑制外周CD4扩张的条件。

DESCRIPTION (adapted from applicant's abstract): The primary aim of this project is to determine if (i) apoptosis of the cycling pool, or (ii) cell cycle arrest is contributing to the failure of CD4 cells to regenerate, and (iii) identify the potential cellular lesions associated therewith. Towards this goal, the investigators will focus on longitudinal samples obtained during the acute phase of anti-viral therapy. They will determine by FACS analysis the presence or intensity of cytokine receptors, adhesion molecules, apoptotic markers, DNA content, and restriction point-related cyclins on Ki67-expressing CD4 cells and their ex vivo cytokine response. By comparing the data obtained at time points which display different kinetics of CD4 lymphocyte regeneration, they hope to better define the conditions which potentiate or inhibit peripheral CD4 expansion.