EXPRESSION, STRUCTURE AND FUNCTION OF FILAGGRIN

Profilaggrin是Profilaggrin的加工形式，是 哺乳动物的终末分化的表皮。基于体外实验 实验中，填充蛋白被认为参与了聚集和 角蛋白中间丝在最后阶段的特异性排列 活体内表皮发育的各个阶段。这一假设一直是 稳定转染角质形成细胞的研究 在可诱导启动子的控制下，一次填充GRIN重复。vt.在.的基础上 启动子的激活，表达的微丝蛋白破坏角蛋白 细胞骨架由于细丝聚集在细胞核附近，导致 细胞的主要形状变化。细胞在6小时内死亡 细胞凋亡。我们已经探索了基因表达的调控。 Profilaggrin基因，并鉴定了其近端启动子的特征。AP1 结合c-fos和c-jun蛋白的位点及其同源物 角质形成细胞特异性表达该基因，与邻近基因一致 SP1、Ets-like和NF-KB元件。我们发现了一种新颖的外星人 一种转录蛋白，它似乎在调节 几个基因在表皮中的表达，包括profilaggrin。

Filaggrin, the processed form of profilaggrin, is a major product of terminally differentiating mammalian epidermis. Based on in vitro experiments, fillaggrin is thought to be involved in the aggregation and specific alignment of keratin intermediate filaments during the final stages of development in the epidermis in vivo. This hypothesis has been explored by stable transfection into keratinocytes of a vector containing one fillaggrin repeat under the control of an inducible promoter. Upon activation of the promoter, the expressed filaggrin disrupts the keratin cytoskeleton due to aggregation of the filament near the nucleus, causes major shape changes in the cells. The cells die within 6 hours by apoptosis. We have explored the regulation of expression of the profilaggrin gene and have characterized the proximal promoter. An AP1 site and its cognate binding c-fos and c-jun proteins confer keratinocyte-specific expression to the gene, in concert with neighboring Sp1, ets-like, and NF-KB elements. We have discovered a novel ets transcription protein that seems to function in the regulation of expression of several genes in the epidermis, including profilaggrin.