NEUROGENESIS AND GLIOGENESIS IN THE DEVELOPING BRAIN

核因子-1和AP-1转录因子在 一系列基因的调节区。因为有可能 核因子-1参与血管内皮细胞表达基因的表达 CNS/PNS，我们筛选了从人新生儿和 检测胎儿脑组织中可能存在的脑特异性核因子-1 带有与DNA结合同源的寡核苷酸探针的因子 核因子-1的结构域。两个来自新生儿脑库的克隆和一个 对从胎脑文库中克隆的基因进行测序。3个测序的cDNA 除了5‘端有几个碱基外，其他克隆都是同源的 两个最长克隆的末端和在3‘-区的150个碱基的插入 最矮的克隆人。DNA结合区位于DNA的5‘端 克隆在大脑克隆之间高度保守，据报道 用于HeLa核因子-1克隆。另一方面，c-DNA的3‘-区 从脑库中分离的克隆高度同源，但 与HeLa细胞报道的结果不同。核因子-1的3‘-区 据报道，分子中含有转录激活因子。 分子的结构域。其中一个脑cDNA克隆被克隆到 构建了T7 RNA聚合酶表达载体，并获得了一个特定大小的蛋白。 IPTG诱导表达时产量过高。准备好的摘录 从细胞中过量生产的特定蛋白质被证明含有 特定的结合活性。为了确定其他类别的核因子- 1可在原代胎儿脑细胞中检测到，RT-PCR分析表明 用来自原代胎儿细胞和HeLa的Poly A选择的RNA进行 细胞。通过使用类特异性引物，至少可以表达 在这两种细胞中都可以检测到四类核因子-1蛋白家族。 脑细胞和HeLa细胞系。然而，在胎脑培养中，有一种 克隆性、核因子-1/AT1的表达高于其他3类。 培养的星形胶质细胞也分析花生四烯酸的表达。 对细胞因子刺激作出反应的代谢产物。在IL-1存在的情况下 人星形胶质细胞产生前列腺素E2和前列腺素F2 阿尔法。环氧合酶途径中的其他中间化合物有 与啮齿动物星形胶质细胞的相似培养方式不同。这两个都是 研究强调了这样一个事实：人类星形胶质细胞具有表型和 神经元型与啮齿类动物模型的基因差异 神经胶质形成。

The NF-1 and AP-1 transcription factors have adjacent binding sites in the regulatory region of a number of genes. Because of the possible involvement of NF-1 with the expression of genes expressed in the CNS/PNS, we have screened cDNA libraries prepared from human neonatal and fetal brain tissue for the possible presence of a brain specific NF-1 factor with an oligonucleotide probe homologous with the DNA binding domain of the NF-1. Two clones from the neonatal brain library and one clone from the fetal brain library were sequenced. The 3 sequenced cDNA clones were homologous with each other, except for a few bases at the 5' end of the two longest clones and a 150-bp insertion in the 3'-region of the shortest clone. The DNA binding region located at the 5'-end of the clones was highly conserved between the brain clones and that reported for the HeLA NF-1 clone. On the other hand, the 3'-region of the c-DNA clones isolated from the brain libraries were highly homologous but differed from that reported from HeLA cells. The 3'-region of the NF-1 molecules were reported to contain the transcriptional activational domain of the molecular. One of the brain cDNA clones was cloned into a T7 RNA polymerase expression vector, and a specific size protein was overproduced on induction of expression with IPTG. An extract prepared from cells overproducing the specific protein was demonstrated to contain specific binding activity. In order to determine if other classes of NF- 1 could be detected in primary fetal brain cells, RT-PCR analysis was performed with poly A-selected RNA from primary fetal cells and HeLa cells. With the use of class-specific primers the expression of at least four classes of the NF-1 protein family could be detected in both the brain and the HeLA cell lines. In the fetal brain cultures, however, one clone, NF-1/AT1 was highly expressed compared with the other 3 classes. Astrocyte cultures were also analyzed for expression of arachidonic acid metabolites in response to cytokine stimulation. In the presence of IL-1 beta, human astrocytes produce prostaglandin E2 and prostaglandin F2 alpha. Other intermediate compounds in the cyclooxygenase pathway are not made unlike similar cultures of rodent astrocytes. Both of these studies highlight the fact that human astrocytes have phenotypic and genotypic differences compared with rodent models of neuro and gliogenesis.