BINDING FORCES IN RECEPTOR MEDIATED CELL ADHESION

由于已知HIV的Tat蛋白将在 体外和刺激产生艾滋病病毒的产品，几乎有 当然，它在细胞膜上有一个受体位置。其中的几个部分 正在合成86种氨基酸蛋白质以确定哪一段 负责与受体结合。这一现象将是 通过定量测量对象之间的约束力进行评估 在组织中生长的各种节段和几种类型的人类细胞 文化。我们正在使用定制的流动单元和图像分析来 确定分离细胞所需的平均剪切力。这是 通过让电池附着在96口井的底部来实现 板材，可在计算机下自动从一个井移动到另一个井 控制力。

Since it is known that the tat protein of the HIV will enter the cell in vitro and stimulate the production of HIV products, there is almost certainly a receptor site for it on the cell membrane. Segments of this 86 amino acid protein are being synthesized to determine which segment is responsible for binding to the receptor. This phenomenon will be evaluated by quantitatively measuring the binding force between the various segments and several types of human cells grown in tissue culture. We are using a custom built flow cell and image analysis to determine the average shear force needed to detach the cells. This is accomplished by allowing the cells to attach to the bottoms of a 96-well plate, which will be moved automatically from well to well under computer control.