ALUMINUM STANDARDS FOR ALZHEIMER'S DISEASE RESEARCH

铝（Al）在土壤中的浓度和分布数据 阿尔茨海默氏病（AD）的标本报告的各个实验室， 由于几个原因很难比较。 其中包括不同的 微量分析技术，样品制备程序，人工制品， 和检测极限。 解释所需的基本信息 并且定量地比较数据，特别是图像数据， 在许多已发表的研究成果中， 样本人群尚未被 根据疾病定义的一致标准进行选择。 因此，在本发明中， 不仅不可能阐明铝在病因学中的作用， 但是，这是不可能的，甚至确定的疾病。 它可能发挥作用的条件。 审查……的问题 人工制品，使用极其严格的 避免铝污染的方法。 这些匀浆中掺杂有 铝，给出高达200 ppm的Al浓度范围。 样品 采用石墨炉原子吸收光谱法（AA）进行分析， 电子束X射线微区分析（EPMA）。 通过AA， 纯鼠脑组织匀浆中的铝含量为一个数量级 低于大多数已发表的数值，但浓度是 大于每个样本组的平均值。 AA和EPMA之间的关系是通过考虑，通过光谱 建模，总样品成分和厚度为电子探针 测量.

Data on the concentrations and distributions of aluminum (Al) in Alzheimer's disease (AD) specimens reported by various laboratories are difficult to compare for several reasons. These include different microanalytical techniques, specimen preparation procedures, artifacts, and detection limits. The fundamental information necessary to interpret and to quantitatively compare data, image data in particular, has been lacking in many published results. Sample populations have not been selected to a consistent standard of disease definition. As a result, not only has it been impossible to clarify the role of Al in the etiology of the disease, but it has been impossible even to determine the conditions under which it may have a role. To examine the issue of artifacts, rat-brain homogenates were prepared using extremely stringent methods to avoid Al contamination. These homogenates were doped with aluminum, giving a range of Al concentrations up to 200 ppm. The samples were analyzed by graphite furnace atomic absorption spectrometry (AA) and electron beam X-ray microanalysis (EPMA). By AA, the concentration of Al in pure rat-brain homogenate was found to be an order of magnitude lower than most published values, but concentrations threefold to tenfold greater than the average for each sample set were found. Good agreement between AA and EPMA was obtained by taking into account, by spectrum modeling, the total sample composition and thickness for EPMA measurements.