ELECTRON MICROPROBE IMAGING AND QUANTITATION OF ALZHEIMER'S ASSOCIATED PLAQUES

阿尔茨海默病相关斑块的电子显微镜成像和定量

• 批准号: 3937319
• 负责人: C R SWYT
• 金额: --
• 依托单位: DIVISION OF RESEARCH SERVICES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3937319
• 关键词: Alzheimer's disease; aluminum; brain; calcium; electron microscopy; electron probe spectrometry; histopathology; human tissue; lyophilization; online computer; organosilicon compound; postmortem; tissue /cell preparation

The post-mortem distributions and concentrations of calcium, silicon, and aluminum in plaques from brains of patients with Alzheimer's disease have been determined from elemental images and spectra acquired by wavelength-dispersive x-ray spectroscopy on a computer-controlled Cameca MBX electron beam x-ray microanalyzer. Both air dried and freeze-dried cryo-sections 8-10 micrometers thick from fresh frozen specimens with no chemical preparation were analyzed. In air-dried sections the concentrations of silicon and aluminum in the plaques were found to be similar to matrix control levels; the concentration of calcium was about fifty per cent higher in the plaques. In freeze-dried sections the concentration of silicon was the same in matrix and most plaques; the concentrations of calcium and aluminum in plaques were found to be up to ten times matrix levels. These results differ from those obtained by other workers using fixed material or purified cores. The implications are that chemical preparation significantly alters the elemental distributions in the sections.

死后钙的分布和浓度， 硅和铝在脑斑块的患者， 阿尔茨海默病已经从元素图像中确定， 通过波长色散X射线光谱法在 计算机控制的Cameca MBX电子束X射线显微分析仪。 空气干燥和冷冻干燥的冷冻切片均为8-10微米 厚的新鲜冷冻标本，没有化学制剂， 分析了 在风干切片中， 发现板中的铝与基质对照相似 水平;钙的浓度约为百分之五十 更高的斑块。 在冻干切片中， 在基质和大多数斑块中硅的含量相同; 发现斑块中钙和铝的浓度 高达十倍的矩阵水平。 这些结果不同于那些 由其他工人使用固定材料或纯化的核心获得。 这意味着化学制剂会显著改变 元素在剖面中的分布。