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ELEMENTAL IMAGING OF ALVEOLAR MACROPHAGES

ELEMENTAL IMAGING OF ALVEOLAR MACROPHAGES
肺泡巨噬细胞的元素成像
批准号：
4705638
负责人：
C R SWYT
金额：
$0.0万
依托单位：
DIVISION OF RESEARCH SERVICES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/4705638
关键词：
X ray spectrometry alveolar macrophages electron microscopy human tissue imaging /visualization /scanning particle

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中文摘要
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英文摘要
Refractile particles are observed by light microscopy (LM) in alveolar lavaged macrophages from patients exhibiting clinical symptoms of asbestosis, silicosis and coal-miner's emphysema. However the elemental composition and location of these particles in the ultrastructure of the macrophages cannot be determined by LM. Elemental x-ray images obtained in the CAMECA MBX microprobe from large fields of whole air-dried macrophages have enabled investigators to correlate particle composition with disease. High resolution images of individual macrophages in ultrathin sections of epon embeded preparations obtained in the Hitachi H700H analytical electron microscope utilizing bright field (BF), energy dispersive x-ray (EDS) and electron energy loss (EELS) signals have revealed both elemental composition and ultrastructural location of the particles.
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  • 批准号：
    3959973
  • 项目类别：
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  • 财政年份：
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  • 负责人：
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  • 项目类别：
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  • 财政年份：
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  • 负责人：
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  • 依托单位：
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