ELEMENTAL IMAGING OF ALVEOLAR MACROPHAGES

肺泡巨噬细胞的元素成像

• 批准号: 4705638
• 负责人: C R SWYT
• 金额: --
• 依托单位: DIVISION OF RESEARCH SERVICES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4705638
• 关键词: X ray spectrometry; alveolar macrophages; electron microscopy; human tissue; imaging /visualization /scanning; particle

Refractile particles are observed by light microscopy (LM) in alveolar lavaged macrophages from patients exhibiting clinical symptoms of asbestosis, silicosis and coal-miner's emphysema. However the elemental composition and location of these particles in the ultrastructure of the macrophages cannot be determined by LM. Elemental x-ray images obtained in the CAMECA MBX microprobe from large fields of whole air-dried macrophages have enabled investigators to correlate particle composition with disease. High resolution images of individual macrophages in ultrathin sections of epon embeded preparations obtained in the Hitachi H700H analytical electron microscope utilizing bright field (BF), energy dispersive x-ray (EDS) and electron energy loss (EELS) signals have revealed both elemental composition and ultrastructural location of the particles.

光镜下观察肺泡上皮细胞内折射颗粒 来自表现出以下临床症状的患者的灌洗巨噬细胞 石棉肺、矽肺和煤矿工人肺气肿。 然而， 这些颗粒的组成和位置在超微结构的 不能通过LM确定巨噬细胞。 元素X射线图像， 来自大视野完整空气干燥巨噬细胞的CAMECA MBX微探针 使研究人员能够将颗粒成分与疾病联系起来。 高分辨率图像中的单个巨噬细胞的切片， 在日立H700 H分析电子显微镜中获得epon包埋制剂 显微镜利用明场（BF），能量色散X射线（EDS）和 电子能量损失（EELS）信号显示， 颗粒的组成和超微结构位置。