CORE--FACS AND RESEARCH ANIMALS

这项拨款中提出的实验在很大程度上依赖于密切的合作 三个PI之间的互动。 核心组件将大大 促进这些互动。 核心B分为3个部分：a） 转基因/敲除小鼠的繁殖，B）维持常规的 转基因和敲除小鼠，和c）流式细胞术。 核心B被设计为提供衰老T细胞受体转基因， 基因敲除小鼠和常规小鼠， 节目 核心B将繁殖和筛选转基因动物（使用FACS 或PCR分析），并根据需要将它们回交以使它们适合 在该计划的各个项目中详细描述的实验。 因为这个过程是非常劳动密集型的，一个集中的设施， 专门执行这项任务是必不可少的。 核心将获得 研究所需的各种年龄的常规、转基因或基因敲除小鼠 个别项目，必要时对其进行检疫， 筛选转基因/敲除基因表达的方法 每种情况下，并将繁殖和回交的小鼠近交系。 的 我们的目标是获得健康的、遗传同质的小鼠， 项目中的调查员。 调查人员将能够获得这些 但在实验期间将单独饲养动物。 有时是漫长的过继转移实验。 这三个项目中的每一个都严重依赖于流式细胞术的使用， 评估各种细胞表面标志物的表达。 外地资产管制系统的费用 核心B中包含分析，因为：1）所有三个项目都将依赖于 （a）在很大程度上依赖于使用共同的外地资产管制系统设施; 上述动物需要使用流式细胞术，以及3）许多 涉及流式细胞术的研究代表了合作的相互作用 在私家侦探中

The experiments proposed in this grant rely heavily on close collaborative interactions amongst the three PIs. The Core component will greatly facilitate these interactions. Core B is divided into 3 components: a) breeding of transgenic/knockout mice, b) maintenance of conventional transgenic and knockout mice, and c) flow cytometry. Core B is designed to provide aged T cell receptor transgenic, gene knockout and conventional mice that are required by all three projects of the program. Core B will breed and screen transgenic animals (using FACS or PCR analysis) and backcross them as necessary to render them suitable for the experiments detailed in the individual projects of the program. Because this process is very labor intensive, a centralized facility specialized to perform this task is essential. The core will obtain the various aged conventional, transgenic or knockout mice needed for the individual projects, quarantine them if necessary, develop standardized procedures for screening for the transgenic/knockout gene expression in each case, and will breed and backcross the mice to inbred strains. The goals are to have healthy, genetically homogeneous mice for the investigators in the project. Investigators will be able to obtain these mice for experiments but will maintain the animals separately during the course of the sometimes lengthy adoptive transfer experiments. Each of the three projects relies heavily on the use of flow cytometry to assess the expression of various cell surface markers. The costs for FACS analysis is included in Core B because: 1) all three projects will rely heavily on the use of the common FACS facility; 2) screening of the aforementioned animals require the use of flow cytometry and 3) many of the studies involving flow cytometry represent collaborative interactions among the PIs.