INHALATION ANESTHETIC EFFECTS ON THE SPINAL DORSAL HORN

我们实验室的一个长期目标是了解 麻醉剂改变脊髓中的感觉处理。最近的工作 我们的实验室已经揭示了麻醉相关的变化， 外周感受野和神经元对非伤害性 感受野刺激本研究项目的具体目标是 使用这些观察到的麻醉效果作为一种工具， 神经递质系统参与脊髓感觉神经元的调节 通过选定的全身麻醉剂进行处理。这些信息将 更好地理解药理学机制， 全身麻醉药改变神经系统功能。 将采用两个单独的方案来回答以下4个问题 问题：1.）什么样的神经递质系统有助于 氟烷抑制感受野大小和神经元对 感受野刷毛2.）的情况。神经递质系统对 安氟醚抑制感受野大小的能力， 增强神经元对刷扫剩余感受野的反应？ 3.）第三章新型吸入麻醉剂地氟烷与氟烷有何不同 和安氟醚对脊髓感觉处理的影响4.）的情况。什么 神经递质系统有助于地氟烷改变 脊髓感觉处理 问题1、2和4将通过研究 全身和/或脊髓给药的神经递质受体 氟烷、恩氟烷和地氟烷诱导的激动剂和拮抗剂 脊髓背角神经元对非伤害性刺激反应的改变 刺激急性大鼠制备物中的外周感受野。 问题3将通过检查地氟烷对 脊髓背角神经元对非伤害性和伤害性感受性反应 场刺激在慢性猫准备。 这些研究应该在受体水平上定义系统， 有助于所选麻醉剂改变脊髓感觉的能力 处理.虽然最终的作用机制是分子性质的， 这项研究的结果将有助于定义神经递质系统， 很可能是麻醉剂的分子作用 会转化为系统效应， 处理感官信息的系统。

A long-term goal of our laboratory is to understand how general anesthetics alter sensory processing in the spinal cord. Recent work in our laboratory has revealed anesthetic related changes in the size of peripheral receptive fields and neuronal responses to non-noxious receptive field stimulation. The specific aim of this research project is to use those observed anesthetic effects as a tool to define what transmitter systems are involved in modulation of spinal sensory processing by selected general anesthetic agents. That information will provide a better understanding of the pharmacologic mechanisms by which general anesthetics alter nervous system function. Two separate protocols will be employed to answer the following 4 questions: 1.) What neurotransmitter systems contribute to the ability of halothane to depress receptive field size and neuronal response to receptive field brushing? 2.) What neurotransmitter systems contribute to enflurane's ability to depress receptive field size while at the same time enhancing neuronal response to brushing of the remaining receptive field? 3.) Does the new inhalation anesthetic desflurane differ from halothane and enflurane in its effects on spinal sensory processing? 4.) What neurotransmitter systems contribute to the ability of desflurane to alter spinal sensory processing? Questions 1, 2, and 4 will be answered by studying the effects of systemically and/or spinally administered neurotransmitter receptor agonists and antagonists on halothane, enflurane and desflurane induced alterations in spinal dorsal horn neuronal responses to non-noxious stimulation of peripheral receptive fields in acute rat preparations. Question 3 will be answered by examining the effects of desflurane on spinal dorsal horn neuronal responses to non-noxious and noxious receptive field stimulation in a chronic cat preparation. These studies should define, at the receptor level, systems that contribute to the ability of selected anesthetics to alter spinal sensory processing. While ultimate mechanisms of action are molecular in nature, results from this study will help to define neurotransmitter systems that are likely to be the mechanism by which molecular actions of anesthetics are translated into systemic effects that alter the ability of the nervous system to process sensory information.