T GONDII--ROLE OF INTRAEPITHELIAL LYMPHOCYTE HOMING

这项合作应用的重点是粘膜的作用， 对条件致病菌弓形虫的免疫力 口头上- 获得性细胞内寄生虫是最常见的CNS感染， 艾滋病患者是先天性感染的主要来源， 新生儿 在过去的两年里，这些研究人员观察到， 膀胱上皮内淋巴细胞（IEL）是重要的 宿主免疫力的调节剂，这种寄生虫CD 8 + IEL的分离自 急性感染小鼠的肠道提供100%的保护， 当过继转移到幼稚 受惠人士他们实验室的初步观察表明 IEL是肠道的家，也许是其他 挑战后的器官 该项目的第一个具体目标是 以确定IEL在肠中的定位是否是一个 诱导宿主免疫的要求。 研究人员将 确定这些IEL是否表达归巢受体， 阻断这些受体会抑制它们诱导保护的能力。 第二个具体目标是确定IEL的流量是否在 肠子 特别是，研究人员将评估是否 IEL提供长期免疫力，这是否与 记忆表型在IEL上的表达。 最后一个目标是 确定迁移性IEL是否可以防止 慢性感染的宿主 总之，这些发现将使 研究人员进一步确定粘膜的免疫功能， 对这种寄生虫的免疫力

This collaborative application is focused on the role of mucosal immunity to the opportunistic pathogen, Toxoplasma gondii. This orally- acquire intracellular parasite is the most frequent CNS infection in those with AIDS and is a major source of congenital infection in the newborn. In the past two years, these researchers have observed that intestinal-derived intraepithelial lymphocytes (IEL's) are important modulators of host immunity to this parasite CD8+ IEL's isolated from the intestine of acutely infected mice confer 100 percent protection against parasite challenge when adoptively transferred into naive recipients. Preliminary observations from their laboratories suggest that the IEL's home to the intestine and, perhaps, traffic to other organs following challenge. The first specific aim of this project is to determine if localization of the IEL's to the intestine is a requirement for the induction of host immunity. The researchers will determine whether these IEL's express homing receptors and whether blocking of these receptors inhibits their ability to induce protection. The second specific aim is to establish if the IEL's traffic outside the intestine. In particular, the researchers will evaluated whether the IEL provide long-term immunity, and whether this is associated with the expression of a memory phenotype on the IEL. The last objective is to determine whether the migratory IEL's can prevent reactivation in the chronically infected host. Together, these findings will allow the researchers to determine, further, the immunologic function of mucosal immunity against this parasite.