ELECTRODES FOR SENSING PEPTIDE NEUROTRANSMITTERS

描述(摘自申请者摘要)：我们建议开发 一种模型系统，可用于设计一种能够 检测体液中的任何肽类神经递质或 纸巾。这样的电极阵列将能够检测到几个 来自同一部位的多肽神经递质。的主要原则。 建议的模型是抗体与 不同的表位特异性，固定在一个功能化的 具有适当形态的导电聚合物基质，具有 待检测的抗原。该导电聚合物将具有 形态，设计为提供最大的表面积 互动。作为模型抗原，我们将使用牛血清白蛋白 (BSA)，对于抗体，我们将使用纯化的亲和池 兔抗血清中的多克隆抗体。同时互动 两种抗体分子中的一种，化学固定在 相邻的聚合物链，与一个抗原分子，将 在聚合物链之间产生一种交联剂，这将导致 基质导电性的可测量变化。 建议的商业应用：不可用。

DESCRIPTION (Adapted from Applicant's Abstract): We suggest developing a model system, which can be used to design an electrode capable of detecting specifically any peptide neurotransmitter in body fluids or tissues. An array of such electrodes will be able to detect several peptide neurotransmitters from the same site. The main principle of the suggested model is a cooperative interaction of the antibodies with different epitope specificity, immobilized on a functionalized conducting polymer matrix which has the appropriate morphology, with an antigen to be detected. The conducting polymer will possess a morphology, engineered to provide the highest surface area for such interaction. As a model antigen, we will use bovine serum albumin (BSA), and for the antibodies, we will use a pool of affinity purified polyclonal antibodies from a rabbit anti-serum. Simultaneous interaction of the two molecules of antibodies, chemically immobilized on the neighboring polymer chains, with one molecule of the antigen, will create a cross-link between the polymer chains, which will induce a measurable change in the conductivity of the matrix. PROPOSED COMMERCIAL APPLICATION: Not available.