REGULATION OF CAPUT EPIDIDYMIS AND EFFERENT DUCTULES

摘要：附睾对雄激素的依赖性取决于 附睾区域以及雄激素的来源 血液中的循环雄激素（CA）与 网液 假设LA、CA、雌激素或其他 网液的成分差异性地调节结构， 附睾头（CE）不同亚区的功能， 输出小管（艾德）。 为了验证这一假设，我们建议研究 雄激素受体，雌激素受体，分泌蛋白的分布， 行政区和行政区各有三个次区域的组织学和病理学资料。 实验将进行：（1）睾丸切除术， 睾酮植入;（2）睾丸外网结扎，有和没有 睾丸激素植入;和（3）睾丸切除术与睾丸激素， 雌二醇植入物和仅雌二醇植入物。 雄激素和雌激素受体将通过定量 和免疫细胞化学/放射自显影方法。 分泌型 蛋白质将通过培养基的凝胶电泳进行分析， 组织将在体外与（35 S）甲硫氨酸一起孵育。 组织学 将包括超微结构检查与 吸收和蛋白质合成。 而前两个实验的结果将揭示 雄激素，特别是CA和LA之间的差异作用， 第三个实验将揭示雌激素的重要性。 总体而言， 研究结果将为不同地区的监管提供见解， 其分泌物在启动 精子成熟

ABSTRACT: The epididymal dependence on androgens differs depending upon the region of the epididymis as well as the source of androgens- circulating androgens (CA) in the blood versus luminal androgens (LA) in the rete fluid. It is hypothesized that LA, CA, estrogens, or other components of the rete fluid differentially regulate the structure and function of different sub-regions of the caput epididymis (CE) and efferent ductules (ED). To test the hypothesis, we propose to study androgen receptors, estrogen receptors, profiles of secretory proteins, and histology in three sub-regions each of the CE and ED. Three experiments will be conducted: (1) Orchiectomy with and without testosterone implants; (2) extratesticular rete ligation with and without testosterone implants; and (3) orchiectomy with testosterone and estradiol implants and estradiol implant only. Both androgen and estrogen receptors will be examined with quantitative and immunocytochemical/autoradiographic methods. Profiles of secretory proteins will be analyzed by gel electrophoresis of the medium in which tissues will be incubated in vitro with (35S) methionine. The histology will include ultrastructural examination of organelles associated with absorption and protein synthesis. Whereas results of the first two experiments will reveal the role of androgens, especially the differential role between CA and LA, those of the third experiment will reveal the significance of estrogens. Overall, results will provide insights on the regulation of different regions of the CE and ED whose secretions play an important role in initiation of sperm maturation.