MINORITY SCIENTIST AWARD AT MINORITY INSTITUTIONS

这个项目的长期目标是发展R.Renee Reams博士 成为一名独立的生物医学调查员，他将使用技术 在选定的研究导师的实验室中学习，以产生科学 这些数据可以用来申请R01研究基金。这项研究 指导将在雷蒙德·F·诺瓦克博士的实验室提供 韦恩州立大学化学毒理学研究所。技术 里姆斯博士所学到的将帮助她定义和研究将 阐明“铅”对哺乳动物系统的作用机制。他 里姆斯博士将在诺瓦克博士的实验室中学习的具体技术 包括原代肝细胞细胞培养以及传代和 细胞系，免疫印迹分析，总RNA和Poly(A)+RNA分离， Northern印迹分析、核运行分析、逆转录酶 聚合酶链式反应、限制性内切酶分析、克隆和DNA测序 分析。这些当代细胞和分子生物学的方法将 由里姆斯博士在佛罗里达农工大学的实验室里用来 检测铅对酪氨酸羟基酶基因表达的影响 肾上腺和神经细胞的信号转导。TH和 PKC酶的调节将通过使用一种 可用于检测蛋白质印迹的商用单抗。基因 TH和PKC的表达将通过总和 Northern印迹法分离Poly(A)+RNA。反转- 转录酶-聚合酶链式反应将用于检测和 证实酪氨酸羟化酶在治疗前后表达增强 铅治疗。

The long term objective of this project is to develop Dr. R. Renee Reams into an independent biomedical investigator, who will use techniques learned in a selected research mentor's laboratory to generate scientific data the an be used to apply for R01 research funding. The research mentoring will be provided in Dr. Raymond F. Novak's laboratory at the Institute of Chemical Toxicology at Wayne State University. Techniques learned by Dr. Reams will help her define and research problems that will elucidate the mechanism of action of "lead" on mammalian systems. he specific techniques that Dr. Reams will learn in Dr. Novak's laboratory include cell culture of both primary hepatocytes as well as passage and cell lines, western blot analysis, total and poly (A) + RNA isolation, northern blot analysis, nuclear run on assays, reverse-transcriptase polymerase chain reaction, restriction analysis, cloning and DNA sequence analysis. These contemporary approaches in cell and molecular biology will be used by Dr. Reams in the laboratory at Florida A&M University to examine the "Effects of Lead on Tyrosine Hydroxylase Gene Expression and signal Transduction in Adrenal and Neural Cells". Expression of TH and modulation of PKC enzymes will be monitored t the level of protein using a commercially available monoclonal antibody to probe western blots. Gene expression of TH and PKC will be detected by the presence of total and poly (A) + RNA isolation using northern blot analysis. Reverse- transcriptase-polymerase chain reaction will be used to examine and confirm the enhanced expression of tyrosine hydroxylase before and after lead treatment.