FT-IR MICROSCOPY OF MINERAL STRUCTURE IN OSTEOPOROSIS

骨质疏松症导致骨折等后遗症是常见的 医疗问题，这往往会导致严重的经济成本， 对生活方式的限制，甚至是死亡。而各式各样的 已经利用技术来尝试防止骨丢失和 由此导致的骨折，关于这种变化的信息很少 治疗前后骨质疏松性骨质的质量。 骨的“质量”，通过骨结构、矿物质特性来衡量 (晶体大小、取向、成分和含量)和基质 含量，是决定骨骼强度的主要因素。这些 研究将评估矿物质和基质成分 骨质疏松症治疗前后的傅立叶变换 变换红外显微光谱分析(FTIRM) 提供有关以下各项决定因素的量化信息 骨骼质量，但建筑除外。FTIRM是一种 一种光学显微镜与红外线耦合的技术 光谱仪允许在10-20秒内检查组织切片 Upsilon m分辨率。骨质疏松症及其防治的FTIRM分析 人类和灵长类动物的骨骼将被用来检验四个假说。 首先，这项研究将研究一系列合成磷灰石以 验证发现，不同类型的替换在 磷灰石晶格对矿物光谱参数的影响 不同的方式。然后是FTIRM、极化FTIRM和二维 FTIRM技术将用于绘制矿物和基质变化的地图 在骨质疏松的板层、骨小梁和骨质骨中。这个 FTIRM将检验的假设是：(I)显著 矿物质和基质含量以及矿物质的差异 骨质疏松症和正常骨骼之间存在成分。(2)场地 骨质疏松症矿物成分和时间依赖的变化 质量取决于骨骼的周转率。(3)差异 氟化钠与钙剂治疗后骨折发生率的比较 那些接受缓释氟化钠和钙治疗的人是由于 形成的矿物和基质质量的差异以及 组成。(4)不同的用药方案不同程度地影响患者的生活质量。 骨质疏松症患者骨质量恢复正常 动物。要考虑的治疗方法包括：双膦酸盐， 雌激素、甲状旁腺素和降钙素。这些FTIRM分析的结果 将提供对骨骼强度丧失的基础的洞察 在骨质疏松症方面，并可能建议哪些治疗方法最有效 令人向往。

Osteoporosis leading to fracture and other sequelae is a common medical problem which often results in serious financial costs, limitations on lifestyle, and even death. While a variety of techniques have been utilized to attempt to prevent bone loss and resultant fracture, there is little information on the change in the quality of osteoporotic bone before and after such treatments. Bone "quality", measured by bone architecture, mineral properties (crystal size, orientation, composition, and content), and matrix content, is a principal factor in determining bone strength. These studies will evaluate the mineral and matrix constituents of osteoporotic bone before and after treatment, using Fourier transform infrared microspectroscopy (FTIRM) to concurrently provide quantitative information about each of the determinants of bone quality with the exception of architecture. FTIRM is a technique in which a light microscope coupled to an infrared spectrometer permits examination of tissue sections at 10-20 upsilon m resolution. FTIRM analyses of osteoporotic and control human and primate bones will be used to test four hypotheses. First, the study will look at a series of synthetic apatites to verify the finding that different types of substitution in the apatite lattice affect the spectral parameters of the mineral in different ways. Then FTIRM, polarization FTIRM, and two dimensional FTIRM techniques will be used to map the mineral and matrix changes in osteoporosic lamellar, trabecular, and osteonal bone. The hypotheses to be tested by FTIRM will be that: (I) Significant differences in both mineral and matrix content and mineral composition occur between osteoporotic and normal bones. (2) Site and time dependent changes in osteoporotic mineral composition and quality depend on the rate of turnover of the bone. (3) Differences in fracture rate between patients treated with NaF and calcium and those treated with slow release NaF and calcium are due to differences in resultant mineral and matrix quality and composition. (4) Different drug regimens variously influence the restoration of "normal,, bone quality in osteoporotic humans and animals. Treatments to be considered include: bisphosphonates, estrogens, PTH, and calcitonin. Results of these FTIRM analyses will provide insight into the basis for the loss of bone strength in osteoporosis, and may suggest which treatments are most desirable.