MOLECULAR DYNAMICS AT THE DENTIN/ADHESIVE INTERFACE

牙本质粘结的目标是双重的，即提取矿物相 而不会破坏胶原蛋白， 矿物与胶粘剂树脂。 如果达到了这个理想，胶原蛋白 脱矿牙本质的纤维将被包围在树脂中， 防止口腔或牙质液的水解作用。 那里 有大量证据表明，这一目标没有实现 研究人员对应该使用什么技术来实现 理想的牙本质/粘合剂粘结。 迄今为止，牙本质/粘合剂粘结主要是使用 结合粘结强度测量的形态分析。 虽然这些分析加强了我们的理解， 关于牙本质/粘合剂界面的化学问题是 无人回应 拉曼光谱是一种光散射技术， 提供了一个“指纹”的分子物种存在于 sample. 当这项技术与显微镜相结合时，化合物- 可以以横向空间分辨率检测特定分子， 大约1 μ m。 尽管背景荧光传统上 限制了拉曼光谱的使用，在这个实验室所做的工作已经 最近表明，可以通过以下方法来避免荧光的干扰： 使用红色激发源。使用显微拉曼光谱和Kr+ 已经用激光绘制了牙本质/混合物/粘合剂界面的化学图谱， 构建了 这个项目的总体假设是，一个前- 处理方案提供了完全的树脂包封 脱矿区中的胶原蛋白将产生持久的牙本质/ 粘合剂粘结。 可以确定最佳的预处理方案 使用互补的化学、形态学和机械数据。 的 本研究的具体目的是：检验牙本质 用自蚀刻底漆制备的涂料将抵抗胶原蛋白塌陷， 粘性树脂可以渗透以这种方式处理的牙本质;测试 假设粘合树脂完全包封胶原 用自酸蚀底漆处理牙本质后暴露的原纤维; 测试自蚀刻底漆提供上级树脂的假设 暴露胶原蛋白的渗透和包封， 用稳定溶剂特别是丙酮处理的蚀刻牙本质 和HEMA;测试自蚀刻底漆提供更强的 牙本质/粘合剂结合与用 稳定溶剂;测试假设，与蚀刻相比， 用稳定溶剂处理的牙本质自蚀刻底漆提供 牙本质/粘合剂结合在暴露于水溶液时稳定， 环境.

The goal of dentin bonding is twofold, i.e. to extract the mineral phase without damaging the collagen and impregnate the voids left by the mineral with adhesive resin. If this ideal were achieved, the collagen fibers of demineralized dentin would be enclosed in resin and thus, protected from the hydrolytic action of oral or dentinal fluids. There is substantial evidence to indicate that this objective is not achieved and researchers disagree on what techniques should be used to accomplish the ideal dentin/adhesive bond. To date, the dentin/adhesive bond has primarily been studied using morphologic analysis in conjunction with bond strength measurement. Although these analyses have enhanced our understanding, numerous questions about the chemistry of the dentin/adhesive interface are unanswered. Raman spectroscopy is a light scattering technique that provides a "fingerprint" for the molecular species present within a sample. When this technique is combined with a microscope, compound- specific molecules can be detected at a lateral spatial resolution of approximately 1mum. Although background fluorescence has traditionally limited the use of Raman spectroscopy, work done in this laboratory has recently shown that interference from fluorescence can be avoided by using a red excitation source. Using micro-Raman spectroscopy and a Kr+ laser a chemical map of the dentin/hybrid/adhesive interface has been constructed. The overall hypothesis of this project is that a pre- treatment regimen which provides complete resin encapsulation of the collagen in the demineralized zone will result in durable dentin/ adhesive bonds. The optimum pre-treatment regimen can be determined using complementary chemical, morphologic, and mechanical data. The specific aims of this study are: test the hypothesis that dentin prepared with self etching primers will resist collagen collapse and that adhesive resin can penetrate dentin treated in this manner; test the hypothesis that adhesive resin completely encapsulates collagen fibrils exposed after treatment of the dentin with self etching primers; test the hypothesis that self-etching primers provide superior resin penetration and encapsulation of exposed collagen in comparison to etched dentin treated with stabilizing solvents, specifically acetone and HEMA; test the hypothesis that self-etching primers provide stronger dentin/adhesive bonds in comparison to etched dentin treated with stabilizing solvents; test the hypothesis that in comparison to etched dentin treated with stabilizing solvents self-etching primers provide dentin/adhesive bonds that are stable upon exposure to aqueous environments.