CORE--TISSUE CULTURE FACILITY

此共享资源的主要功能是管理 癌细胞系库，并提供必要的组织培养 支持癌症研究人员。该机构提供共享组织 文化设施齐全，维护良好。癌细胞 培养细胞系并提供给研究者， 生长或冷冻细胞库。补充冷冻细胞储备液 按要求细胞系的DNA指纹图谱用作质量 控制措施癌细胞系储存库通过以下方式管理： 完整的库存系统。的单个细胞系， 存储库在计算机数据库中描述。许多组织 提供与培养相关的服务，包括支原体检测， 辅助病毒拯救测定，用于遗传学的血液样品的处理 筛选，小牛血清活性炭吸附，细胞培养，细胞 冷冻、细胞系DNA指纹图谱和生长曲线 分析新批次的小牛血清和胎牛血清。的 从正常乳腺组织和从乳腺癌组织中产生原代细胞系 乳腺癌原发性或转移性肿瘤是一项发展中的服务。 组织培养共享资源是由所有的成员使用， CCSG的程序，它与其他八个中的五个相互作用 共享资源。超过82%的共享资源的使用， 过去的一年是癌症中心成员的审查项目。

The primary functions of this shared resource are to manage the Cancer Cell Line Repository and to provide essential tissue culture support to cancer researchers. The facility provides shared tissue culture areas that are well equipped and maintained. Cancer cell lines are cultured and made available to investigators as either growing or frozen cell stocks. Frozen cell stocks are replenished as required. DNA fingerprinting of cell lines is used as quality control measure. The cancer cell line repository is managed through a complete inventory system. The individual cell lines of the repository are described in a computer database. A number of tissue culture related services are offered, including mycoplasma testing, helper virus rescue assays, processing of blood samples for genetic screening, charcoal stripping of calf serum, cell culture, cell freezing, DNA fingerprinting of cell lines, and growth curve analysis of new lots of both calf serum and fetal calf serum. The production of primary cell lines from normal breast tissue and from breast cancer primary or metastatic tumors is a developing service. The Tissue Culture Shared Resource is used by members of all of the programs of the CCSG, and it interacts with five of the other eight shared resources. Over 82% of the usage of the shared resource in the past year was by -reviewed projects of Cancer Center members.