CORE--LABORATORY CORE

STD-CRC实验室核心将：1)提供必要的专门技术 为研究人员提供的微生物学和实验室专业知识 STD-CRC的临床、流行病学和行为项目；2) 促进基础科学新进展的快速应用或 分子生物学对相关临床和流行病学项目的影响 STD-CRC；3)作为国家和国际参考 实验室供其他研究性传播疾病的研究人员使用；以及4)保持 淋病登记处将继续评估与以下疾病相关的因素 再次感染淋病奈瑟菌。提供的实验室专业知识 将由高度专业化的实验室测试组成，这些测试 即使在其他研究实验室中也不是常规可用的，但 这对拟议的流行病学或临床研究的成功是不可或缺的。 这类检测的例子包括沙眼衣原体血清型的测定； 淋球菌缺陷型和血清型的测定.聚合酶链式反应和LCR试验 脉冲场凝胶电泳法(PFGE)分析N。 淋病；选定衣原体菌株的MOMP核苷酸序列；以及 沙眼衣原体微量IF血清学检测。提供这些测试和其他测试 在中央核心实验室确保它们将由以下人员执行 技术经验丰富的调查人员，通过规模经济降低成本， 并将这些测试提供给进行行为和 流行病学研究，否则可能无法进入实验室 合作者。这种可获得性将促进微生物的使用 行为研究中的终点。 虽然实验室核心的目的不是支持基本的 科学工作，核心将积极寻求调剂适当 从基础科学实验室到相关的临床和 流行病学项目。例如，我们提出了进一步发展的建议 和基于PFGE的淋球菌分型系统的实施，该系统将 即使在相同的生长型和菌株之间也表现出异质性 Serovar，为演示菌株提供了一个更强大的工具 多样性。在实验室核心内执行的程序将是 作为国家和国际资源提供给其他人 调查人员，消除了他们开发昂贵和 技术要求很高的测试。

The STD-CRC laboratory core will: 1) provide necessary specialized microbiological and laboratory expertise for investigators performing clinical, epidemiological and behavioral projects within the STD-CRC; 2) facilitate the rapid application of new advances in basic sciences or molecular biology to relevant clinical and epidemiologic projects within the STD-CRC; 3) serve as a national and international reference laboratory for other investigators studying STDs; and 4) maintain a gonorrhea registry to continue assessment of factors associated with reinfection with Neisseria gonorrhoeae. The laboratory expertise provided in the core will consist of highly specialized laboratory tests that are not routinely available even in other research laboratories but are integral to the success of proposed epidemiological or clinical research. Examples of such tests include determination of C. trachomatis serovars; determination of gonococcal auxotypes and serotypes; PCR and LCR tests for chlamydia; Pulse field gel electrophoresis (PFGE) analysis of N. gonorrhoeae; MOMP nucleotide sequences of selected chlamydia strains; and Micro IF serology for C. trachomatis. Provision of these and other tests in a central core laboratory ensures that they will be performed by technically experienced investigators, reduces costs by economy of scale, and makes these tests available to researchers conducting behavioral and epidemiological studies who might otherwise not have access to laboratory collaborators. Such availability will foster the use of microbiological endpoints in behavioral studies. While it is not the purpose of the laboratory core to support basic science work, the core will actively seek to transfer appropriate technology from basic science laboratories to relevant clinical and epidemiological projects. For example, we propose the further development and implementation of a PFGE based typing system for gonococci that will demonstrate heterogeneity even among strains of the same auxotype and serovar, providing a much more powerful tool for demonstrating strain diversity. The procedures performed within the laboratory core will be available as a national and international resource for other investigators, eliminating the need for them to develop expensive and technically demanding tests.