GENETIC AND PHYSICAL MAPPING RESOURCES

描述：（改编自申请人的摘要）Lander博士和 同事们建议构建所需的关键映射资源， 大鼠作为研究遗传基础的系统的优势 生理学和疾病。 具体而言，它们将： 构建由6,000个简单序列长度组成的大鼠遗传图谱 使用两个F2互交进行多态性（SSLPs）基因分型; 描述48个重要品系大鼠SSLP的等位基因大小 用于遗传学和生理学研究，从而确定 对任何特定的杂交都有意义的标记。 在（SHR X BN）重组近交（R1）品系中对约1，000个SSLP进行基因分型。 确定初始基因组扫描的筛选集，包括400个 均匀间隔的、高度多态的和极其稳健的标记。 确保通过电子数据库迅速分发数据， 服务器. 确保方便和负担得起的获得SSLP的PCR引物， 通过与Research Genetics，Inc. 通过FISH定位，将遗传图谱锚定在细胞遗传图谱上。 在酵母人工染色体（YACs）中构建大鼠基因组文库， 提供7倍覆盖，平均插入片段大小至少为800 kb。 细菌人工染色体中大鼠基因组文库的构建 （BAC），提供7倍覆盖，平均插入大小为约 130kb。 构建两组辐射杂合（RH）细胞系，一组高辐射杂合（RH）细胞系。 包含大片段的连接面板，以及高分辨率 含有小片段的板，允许快速基因组定位 序列，即使多态性不可用。 对约1000个间隔良好的基因座的高连接性RH面板进行基因分型。 确保YAC库、BAC库和RH面板可用 通过与Research Genetics，Inc.

DESCRIPTION: (Adapted from the Applicant's Abstract) Dr. Lander and colleagues propose to construct key mapping resources needed to take full advantage of the rat as a system for the study of the genetic basis of physiology and disease. Specifically, they will: Construct a rat genetic map consisting of 6,000 simple sequence length polymorphism (SSLPs) genotyped using two F2 intercrosses; Characterize the allele sizes of the SSLPs in 48 important rat strains used in genetic and physiological studies -- thereby identifying the markers that are informative for any particular cross. Genotype about 1,000 SSLPs in (SHR X BN) recombinant inbred (R1) strains. Identify a screening set for initial genome scans, consisting of 400 evenly spaced, highly polymorphic, and extremely robust markers. Ensure rapid distribution of data through electronic databases and servers. Ensure convenient and affordable access to PCR primers for the SSLPs, through a distribution arrangement with Research Genetics, Inc. Anchor the genetic map in the cytogenetic map, by using FISH mapping. Construct a rat genomic library in yeast artificial chromosomes (YACs), providing 7-fold coverage with average insert size of at least 800kb. Construct a rat genomic library in bacterial artificial chromosomes (BACs), providing 7-fold coverage with average insert size of about 130kb. Construct two panels of radiation hybrid (RH) cell lines, a high connectivity panel containing large fragments, and a high resolution panel containing small fragments allowing rapid genomic localization of sequences even when a polymorphism is not available. Genotype the high connectivity RH panel for about 1000 well-spaced loci. Ensure availability of the YAC library, BAC library, and RH panels through a distribution arrangement with Research Genetics, Inc.