Functional studies of tyrosine sulfation using synthetic sulfoproteins

该项目旨在解决缺乏知识的酪氨酸残基的翻译后硫酸化如何影响蛋白质功能。该项目将开发一个合成平台，用于快速有效地生成位点选择性硫酸化蛋白质文库。新方法将用于研究蜱分泌的生物活性硫酸化蛋白质，这些蛋白质抑制炎症反应并防止血液凝固。在获得合成磺蛋白的能力的基础上，预期成果包括详细了解酪氨酸硫酸化如何调节具有抗凝和抗炎活性的抗体和蛋白质的功能和稳定性。该项目的重大利益将包括制备均质改性蛋白质的突破性技术，这将加强澳大利亚不断增长的生物技术部门。

This project aims to address a lack of knowledge about how post-translational sulfation of tyrosine residues influences protein function. The project will develop a synthetic platform for the rapid and efficient generation of libraries of site-selectively sulfated proteins. The new methods will be used to study bioactive sulfated proteins secreted by ticks that dampen the inflammatory response and prevent blood from clotting. Underpinned by the ability to access synthetic sulfoproteins, expected outcomes include a detailed understanding of how tyrosine sulfation can modulate function and stability of antibodies and proteins with anticoagulant and anti-inflammatory activities. Significant benefits of the project will include breakthrough technologies for the preparation of homogeneously modified proteins, which will strengthen Australia’s growing biotechnology sector.