RUTHENIUM (II) COMPLEXES AS PROBES OF INTRACELLULAR (INTRA ENDOCYTIC) OXYGEN

我们测量了细胞内和细胞外的氧浓度， 小鼠单核细胞-巨噬细胞培养物，J774A.1，使用Ru（II） 配合物[Ru（bpy）3]2+和[Ru（phen）3]2+的荧光寿命 显微镜 利用时间分辨技术我们可以比较 对空气和氮气之间氧浓度的响应 气氛 氧依赖的相位角变化， [Ru（bpy）3]2+和[Ru（phen）3]2+在细胞内和细胞外均存在。 在空气和氮气之间的10-12度（400 kHz）的量级。 的 显微镜下的空间分辨率处于衍射极限， 这使我们能够测量氧气浓度， 单个12微米大小巨噬细胞。

We measured intra- and extra-cellular concentrations of oxygen in Mouse Monocyte-Macrophage cultures, J774A.1, using the Ru(II) complexes [Ru(bpy)3]2+ and [Ru(phen)3]2+with the fluorescence lifetime microscope. Using the time-resolved technique we were able to compare response for oxygen concentrations between air and nitrogen atmosphere. The oxygen-dependent changes in phase angle for [Ru(bpy)3]2+ and [Ru(phen)3]2+ inside and outside cells were in the order of 10-12 degrees (400 kHz) between the air and nitrogen. The spatial resolution under the microscope was at the diffraction limit, which allowed us to measure the concentration of oxygen inside a single 12 micrometer size macrophage.