DIET FRUCTOSE AND GLYCATING COMPOUNDS IN HUMAN RED CELLS

蔗糖和高果糖形式的精制糖的消耗 玉米糖浆在美国和西方世界的含量非常高。而 已经研究了这种现象对公共卫生的影响 广泛地说，最近发现的果糖-3-磷酸（F3 P）在人类 红细胞提示应检查人的果糖代谢 此外，特别强调这种新的代谢途径。等 需要进行一项研究，因为初步数据表明红细胞F3 P 在人类中，F3 P主要由膳食果糖产生， 有效反应性酮醛3- 脱氧葡萄糖醛酮（3DG）。这种化合物被认为是一把钥匙这把钥匙 蛋白质非酶糖化过程中的中间体， 似乎与糖尿病并发症的病因有关。 已发表的证据表明，这种有毒化合物是在体内产生的 在动物和人类中，通过还原为3-脱氧果糖而解毒 （3DF）。 本研究旨在探讨果糖-3-酮的合成细节， 在离体和体内的人红细胞中， 强调了解解毒机制及其可能的 病理状况如高血糖症的损害。

Consumption of refined sugars in the form of sucrose and high-fructose corn syrup is very high in the U.S. and in the Western world. While the public health implications of this phenomenon have been studied extensively, the recent discovery of fructose-3-phosphate (F3P) in human erythrocytes suggests that fructose metabolism in man should be examined further, with a particular emphasis on this novel metabolic pathway. Such a study is needed because preliminary data suggest that erythrocytic F3P in man is produced primarily from dietary fructose and that F3P is an important systemic source of a potent reactive ketoaldehyde 3- deoxyglucosone (3DG). This compound is considered to be a key this a key intermediate in the process of non-enzymatic glycation of proteins which appears to be involved in the etiology of diabetic complications. Published evidence suggests that this toxic compound is produced in-vivo in animals and man and is detoxified by reduction to 3-deoxyfructose (3DF). This proposal seeks to investigate the details of synthesis of fructose-3- phosphate in human erythrocytes ex vivo and in vivo with a particular emphasis on understanding the detoxification mechanisms and their possible impairment in pathological conditions such as hyperglycemia.