NOVEL APPROACHES TO IMPROVE AND EXTEND PLATELET STORAGE

提出了一种新的提高保存血小板质量的方法 在任何储存间隔，并延长血小板储存时间。 将对广泛的储存条件进行评估。 将检验两个主要假设，它们可能解释渐进性 储存过程中血小板活性的丧失：1)代谢需要 在储存过程中不满足血小板；和/或2)血小板满足 在收集、处理和存储期间逐渐激活 在输血后导致快速去除血小板的血小板。 研究计划测试输血后的生存能力和功能。 储存在为满足代谢需求而设计的溶液中的血小板 在添加或不添加激活抑制剂的情况下的血小板。 血小板的长期储存将使用已经被证实的试剂进行评估 记录以维持各种细胞和组织的完整性 在冷冻保存和/或冷冻干燥(冷冻干燥)期间。其他 方法包括将血小板加热到37摄氏度，持续一小时 输血便于修复任何可逆的储存诱导 以前导致立即移除受损的 循环中的血小板数量。我们还将收集和 储存来自之前接受过血液分离的捐献者的血小板 促血小板生成素(MGDF)增加他们的血小板计数。它是 假设从这些MGDF刺激的捐赠者身上采集的血小板 将包含更年轻的血小板数量，可能比 在液体储存期间和/或在 冷冻保存或冷冻干燥。 几种新的仪器和分析方法可能更好地预测后 输注后的血小板活性和功能比目前可用的 体外测试将测试它们与后两者的相关性。 输注血小板回收率、存活率和出血时间的测量。

Novel approaches are proposed to improve the quality of stored platelets at any storage interval and to extend the duration of platelet storage. A broad spectrum of storage conditions will be evaluated. Two major hypotheses will be tested that may explain the progressive loss of platelet viability during storage: 1) the metabolic needs of platelets are not being met during storage; and/or 2) platelets are progressively activated during the collection, processing, and storage of platelets leading to rapid platelet removal following transfusion. Studies are planned to test the post-transfusion viability and function of platelets stored in solutions designed to meet the metabolic needs of platelets with or without the addition of activation inhibitors. Long-term platelet storage will be evaluated using agents that have been documented to maintain the integrity of a variety of cells and tissues during cryopreservation and/or freeze-drying (lyophilization). Other approaches involve warming platelets to 37 C for one hour prior to transfusion to facilitate repair of any reversible storage-induced lesions that previously resulted in the immediate removal of a damaged population of platelets from circulation. We will also collect and store platelets from apheresis donors who have been previously given thrombopoietin (MGDF) to increase their platelet counts. It is hypothesized that platelets harvested from these MGDF-stimulated donors will contain a population of younger platelets that may be more resistant to injury during both liquid storage, and/or during cryopreservation or freeze-drying. Several new instruments and assays that may better predict post- transfusion platelet viability and function than the currently-available in vitro assays will be tested for their correlation with post- transfusion platelet recovery, survival, and bleeding time measurements.