ENZYME-MEDIATED ANALYTICAL ASSAYS

为提高最广泛使用的酶介导分析方法的灵敏度、信噪比和精密度，提出了新技术的发展。除此之外，它还可以用于显著减少分析时间和节省昂贵的材料。使用模型系统的初步结果证明了所提出的技术的潜力：与传统方法相比，扩增方法可以将酶检测的灵敏度提高10-20倍。分析时间也可以减少到原来的1/3-1/5。由于这项技术是基于一般方法的使用，它不仅可以用于免疫分析，还可以用于其他酶介导的分析生化方法。我们建议进一步改进和优化新技术，以产生适合临床实践的常规技术。我们希望这一发展将为检测体液中的生理活性物质提供成本更低的方法。拟议的商业应用：疾病的诊断越来越需要有效和准确地定量测定体液中的生理活性物质(有关市场分析的详细信息，请参阅第20页)。与传统的分析方法相比，使用所提出的方法进行分析的成本应该要小得多。我们的扩增方法具有灵敏度高、分析时间短、节省昂贵材料等优点，使我们相信基于该技术制造的新产品具有巨大的商业潜力。

Development of new technology is proposed for increase the sensitivity, signal-to-noise ratio and precision of most widely used enzyme-mediated analytical assays. Except that, it can be employed for significant reducing the time of analysis and sparing costly materials. Preliminary results using model systems demonstrated the potential of the proposed technology: the amplification method allowed increasing the sensitivity of the enzyme detection 10-20-fold, compared to conventional procedures. It was also possible to reduce time of analysis 3-5-fold. Since this technology is based on the use of a general approach, it can be employed not only for immunoassays but other enzyme-mediated analytical biochemical methods. We propose to further improve and optimize the new technology, so as to generate routine technique suitable for clinical practice. We expect this development will provide less expensive method for the detection of physiologically active substances in body fluids. PROPOSED COMMERCIAL APPLICATIONS: The diagnosis of diseases is increasingly demanding the efficient and accurate quantitative determination of physiologically active substances in body fluids (see page 20 for details on market analysis). The cost of analysis using the proposed methods is supposed to be significantly smaller, compared to that for the conventional assays. The availability of advantages of our amplification method such as increase in the sensitivity, shortening the time of analysis and sparing of costly materials allow us to believe that the new products manufactured on the basis of this technology have a great commercial potential.