GLASS BASED BIOCHIP AND OPTICAL DETECTOR

新维度研究和仪器(NDR)计划制造一个基于玻璃的生物芯片系统，这是一个获取、分析和管理复杂遗传信息的独特平台。这种创新的基于玻璃的芯片及其相关的芯片读取器能够使用分子信标染料和矩阵可寻址电场实时监控DNA杂交，以精确控制化学反应。基于玻璃的生物芯片采用了可自寻址、自组装的微电子系统，可以以微观形式主动进行和控制多步骤和多重反应。通过使用我们的分子信标，只有完全匹配的DNA对才会发出荧光，(不匹配的对将不会荧光)，我们可以实时监控杂交过程。此外，还将消除杂交过程中的化学清洗。我们将展示和开发一种检测低拷贝数核酸靶标的方法和装置，它结合了1)用于选择性杂交的高特异性分子信标，2)靠近荧光探针的高灵敏度检测器，以及3)用于选择性杂交的电场。拟议的商业应用：生物芯片技术使研究人员和临床医生能够比以往任何时候都更快、更有效地分析大量的遗传信息，可以精确地确定感染、非感染和遗传性疾病。该系统已被开发为一种工具，用于帮助了解人类基因组，并改进对癌症、结核病、艾滋病毒、乙肝等疾病的诊断、监测和治疗。

New Dimension Research and Instrument (NDR), proposes to make a glass-based BioChip system, a unique platform for acquiring, analyzing and managing complex genetic information. This innovative glass-based chip, and its associated chip reader are able to real- time monitor DNA hybridization by using molecular beacon dye and a matrix-addressable electrical field for precise control of chemical reactions. The glass-based BioChip uses a self-addressable, self- assembling micro-electronic system, which can actively carry out and control multi-step and multiplex reactions in microscopic formats. By using our molecular beacon in which only exactly matched DNA pairs will be fluorescent, (unmatched pairs will not fluoresce), we can real-time monitor the hybridization process. Moreover, the chemical washing in the hybridization process will be eliminated. We will demonstrate and develop a method and a device for detecting low copy numbers of nucleic acid target by combining 1) high specificity of molecular beacon for selective hybridization, 2) high sensitivity detector near the fluorescent probe, and 3) electric field for selectivity hybridization. PROPOSED COMMERCIAL APPLICATION: BioChip technology enables researchers and clinicians to analyze large amounts of genetic information more rapidly and efficiently than was ever before possible, which can precisely determine infection, non-infection, and genetic diseases. The system has been developed as a tool to help understand the human genome and to improve the diagnosis, monitoring and treatment of diseases, such as cancers, TB, HIV, HB, etc.