CORE--MOLECULAR BIOLOGY

分子生物学技术已经彻底改变了 生殖生物学家解决特定的生理问题， 快速，敏感的方式。然而，这种方法通常需要大量的 为获得适当的专门知识和设备而投入的时间， 在快速发展的领域保持最新的时间承诺，以及 即使是最常规的质量控制， 重复的技术。许多分子实验的主要成本是 提供可靠的试剂和探针的劳动力。一个集中的， 装备精良的核心是最有效和最经济的方式， 重组DNA方法和试剂的项目，以追求创新 生殖医学的分子研究分子的目标 核心是为研究项目提供服务、专业知识和设备 解决生殖方面的临床和基本问题。三个研究 U54内的项目，以及五个额外的项目， 生殖科学分支需要访问核心进行常规操作 探针标记服务（末端标记、随机引物和单链 RNA核糖核酸探针），DNA分离，质粒生长和纯化，DNA 测序和DNA片段分离和亚克隆程序。核心 主任和工作人员将提供咨询和专业知识的设计， 探针和表达载体，各种RNA和DNA定量方法， 和专门技术，如文库筛选。核心人员将 提供及时、质量受控的服务和试剂， 项目优先。调查人员可以进入核心 专门的设备，以执行更先进的技术， 可用性基础。最后，核心人员将继续改进， 根据项目的需要开发额外的服务和程序 访问核心，并更新现有服务，以提供最 灵敏、精确、经济的试剂。

Molecular biology techniques have revolutionized the ability of reproductive biologists to address specific physiological questions in a rapid, sensitive manner. This approach, however, often requires a large time investment to acquire appropriate expertise and equipment, additional time commitments to keep current in a rapidly advancing field, and critical attention to quality control for even the most routine and repetitive techniques. A major cost for many molecular experiments is the labor involved to provide dependable reagents and probes. A centralized, well-equipped core is the most efficient and economical way to provide recombinant DNA methodology and reagents for projects to pursue innovate molecular work in reproductive medicine. The objective of the Molecular Core is to provide services, expertise, and equipment to research projects addressing clinical and basic questions in reproduction. Three research projects within the U54, and five additional projects approved by the Reproductive Science Branch require access to the Core for routine services of probe labeling (end-label, random priming, and single-strand RNA riboprobes), DNA isolation, plasmid growths and purification, DNA sequencing, and DNA fragment isolation and subcloning procedures. The core director and personnel will provide advice and expertise on the design of probes and expression vectors, various RNA and DNA quantitation methods, and specialized techniques such as library screening. Core personnel will provide timely, quality-controlled service and reagents to approved projects on a priority basis. Approve investigators can access the core for specialized equipment to perform more advanced techniques on a availability basis. Finally, Core personnel will continue to improve and develop additional services and procedures as required by projects accessing the core, and update current services to provide the most sensitive, precise, and cost-effective reagents.