MEIOTIC GENETICS IN D DISCOIDEUM

其唯一目标是开发一种减数分裂遗传分析系统 盘基网眼菌。网柄网柄菌已成为一种模式系统 为研究单细胞阿米巴运动和趋化性以及多细胞阿米巴 细胞命运的选择和调控。主要的限制是 这种单倍体生物体的遗传分析的困难。 近些年来，由于Dictyostelius容易 同源基因置换研究多种细胞功能。 在野外，网柄网柄藻有一个有性的二倍体周期。不幸的是， 网柄网柄菌的实验室菌株已经失去了这种能力。凯辛 提出了建立网柄菌有性品系的方法，这将 对于遗传作图、菌株的同源性和创造 多个突变种群。

The single aim is to develop a system of meiotic genetic analysis for Dictyostelium discoideum. Dictyostelium has served as a model system for studying single cell amoeboid motility and chemotaxis and multi- cellular fate selection and regulation. The major limitation has been the difficulty in genetic analysis of this haploid organism. Dictyostelium has enjoyed a resurgence in recent years due the ease of homologous gene replacement to study a variety of cellular functions. In the wild, Dictyostelium has a sexual, diploid cycle. Unfortunately, the laboratory strains of Dictyostelium have lost this ability. Kessin proposes methods to establish sexual strains of Dictyostelium which will be essential for genetic mapping, isogenization of strains, and creating multiple mutant stocks.