GENETIC MODEL OF ENDURANCE EXERCISE IN RATS

描述(改编自申请人的摘要)：长期目标 这项工作是为了识别决定Low和Low之间差异的基因 哺乳动物的高耐力表现。第一步，如这里所示， 是培育出不同品系的老鼠，这些品系的表达达到了极端的 通过选育的耐力跑。确定了两项初步研究 低耐力跑合和高耐力跑合的选育参数 老鼠。首先，对跑步机耐力跑的遗传力进行了估计 Spraogue-Dawley大鼠小实验种群(n=48)。距离跑到 精疲力竭被用来衡量耐力。两对最高的和 最低的是选择性繁殖的。优选者和低选者的后代 老鼠在跑步表现上有29.4%的差异 第一代，遗传力(H2)狭义为0.50。 其次，测定了192只N/NIH大鼠的耐力跑成绩。 在表现最好的情况下选择配对的目的。这个 选择N/NIH是因为它们具有广泛的遗传异质性。十三 交配对是在表现最好的情况下挑选出来的，并展示了 广泛的选择差异。13名被选得最高的女性平均参加 比13只低选雌性的距离长3.55倍。13个高选 雄性奔跑的距离是低级雄性的5.59倍。这些数据预示着 大鼠跑步耐力表现的育种价值。具体目标 这项工作的目的是：1.创造出低耐力和高耐力的品系 选育N/NIH大鼠直到遗传为止的生产性能 停滞不前。2.继续选育小试 在遗传高原之前，Spraogue-Dawley大鼠的数量。 低、高差异基因的鉴定 运动耐力将为理解遗传基因奠定更广泛的基础 健康和疾病的起源。例如，低性能选定的大鼠 可能集中了决定心力衰竭发展的基因或 寿命缩短。此外，这些运动表现模型还可以 作为#年拟议的“生物体项目”的中心组成部分 功能基因组学。

DESCRIPTION (Adapted from the applicant's abstract): The long term goal of this work is to identify genes that dictate the difference between low and high endurance performance in mammals. The first step, as presented here, is to develop divergent strains of rats that express to the extremes of endurance running by selective breeding. Two preliminary studies defined parameters for selective breeding for low and high endurance running in rats. First, heritability of treadmill endurance running was estimated in a small test population (n=48) of Sprague-Dawley rats. The distance run until exhaustion was used as a measure of endurance. Two pairs of the highest and lowest were selectively bred. The offspring of the high and low selected rats demonstrated a 29.4 percent difference in running performance in the first generation and had a narrow sense of heritability (h2) of 0.50. Second, the endurance running performance of 192 N/Nih rats was measured for the purpose of selective breeding pairs at the extremes of performance. The N/Nih were chosen because they have wide genetic heterogeneity. Thirteen mating pairs were selected at the extremes of performance and demonstrated a wide selection differential. The 13 high selected females ran on average 3.55 times further than the 13 low selected females. The 13 high selected males ran 5.59 times further than the low males. These data predict a high breeding value for running endurance performance in rats. The specific aims of this work are to: 1. Create strains of low and high endurance performance rats by selective breeding of the N/Nih rats until inheritance plateaus. 2. Continue selection and breeding of the smaller test population of Sprague-Dawley rats until inheritance plateaus. Identification of genes responsible for the differences between low and high exercise endurance would form a broader base for understanding the genetic origins of health and disease. For example, low performance selected rats may concentrate genes that determine the development of heart failure or reduced longevity. In addition, these models of exercise performance could serve as a central component of the proposed "Physiome Project" for functional genomics.