STUDIES OF MOLYBDENUM CONTAINING ENZYMES

目前的应用包括对四种相关的单核钼酶的基本酶学和光谱性质的综合研究：黄嘌呤氧化还原酶、亚硫酸盐氧化酶、DMSO还原酶和亚砷酸还原酶。该组包括所有三个主要的单核钼酶家族的代表。所提出的工作的总体目标是通过比较和对比这些酶的物理、化学和电子结构，对每一种酶的作用机制的基本方面有更全面的了解。本文还将比较不同类型酶的结构与功能的关系。为此，一系列快速反应动力学、光谱学、理论和分子生物学研究将直接建立在PI实验室过去的工作基础上。所提出的工作旨在将这些酶的机制和光谱研究与已知的晶体结构结合起来，并通过比较和对比它们的性质来阐明反应性的潜在主题。这种方法已被证明是非常成功的研究，一般更了解的生物系统，拥有血红素和黄素假体基团。

The present application involves a comprehensive investigation of the basic enzymological and spectroscopic properties of four related mononuclear molybdenum enzymes: xanthine oxidoreductase, sulfite oxidase, DMSO reductase and arsenite reductase. This group includes representatives of all three major families of mononuclear molybdenum enzymes. The overall goal of the proposed work is to gain a more complete understanding of fundamental aspects of the mechanism of action of each of these enzymes by comparing and contrasting their physical, chemical and electronic structures. A comparison among enzymes type with regard to the relationship of structure to function will also be made. To this end a series of rapid reaction kinetic, spectroscopic, theoretical and molecular biological studies will be undertaken that build directly on past work in the PI's laboratory. The proposed work is intended to integrate both mechanistic and spectroscopic studies of these enzymes with their known crystal structures and to elucidate underlying themes in reactivity by comparing and contrasting their properties. This approach that has proven extremely successful in studies of the generally much better understood biological systems that possess heme and flavin prosthetic groups.