60-HZ MAGNETIC FIELDS AND HORMONE LEVELS IN WOMEN

拟议研究的主要目标是确定 家庭睡眠环境中暴露在60赫兹磁场中 由电热毯产生的是与减少 夜间尿褪黑素水平与夜间尿量增加 黄体生成素(LH)、卵泡刺激素(FSH)、 在健康育龄妇女的样本中检测到雌二醇。 其次，这项研究旨在调查两者之间的关系 夜间尿褪黑素水平与夜间尿液水平的关系 促黄体生成素、促卵泡生成素和雌二醇，而不考虑夜间的磁暴露 菲尔兹。至少180名妇女将被招募为志愿者 西雅图大都市区。有资格参加的将是任何女性 年龄在20岁到45岁之间，是国王县的居民，他有 以前没有被诊断出患有乳腺癌，世卫组织定期报告 月经期间，谁没有服用口服避孕药或激素 准备，谁通常睡在电热毯在 冬天的几个月。这项调查将采用交叉设计 它将每个参与者视为她自己的控制。数据收集将 发生在冬季的几个月里，当电热毯 通常使用，每个需要三个月的时间 主题。第一个月用来评估月经周期。 规律性。第二个月和第三个月分别由冲刷组成 曝光期之后是曝光期(电热毯使用 从排卵日开始到排卵后第7天)或非 暴露期(从排卵日开始不使用电热毯 至第七天后评估)。一半的受试者将被分配到 暴露后不暴露，而另一半受试者 将按相反的顺序分配，先不曝光，再曝光。 使用商业试剂盒，将确定每个月的排卵量。 在第2个月和第3个月，磁场水平将在 每个受试者从排卵后第3天到 排卵后第7天，夜间尿液将在第7天采集 排卵后。尿样将被化验为初级尿液 褪黑素代谢产物6-硫代谢素及血清促黄体生成素、卵泡刺激素、 和雌二醇。关于受试者的病史、用药情况的信息 使用情况，还将收集电热毯的特性。

The primary objective of the proposed study is to determine whether exposure to a 60-Hz magnetic field in the home sleeping environment produced by an electric blanket is associated with a decrease in nocturnal urinary melatonin levels and an increase in nocturnal urinary levels of luteinizing hormone (LH), follicle stimulating hormone (FSH), and estradiol in a sample of healthy women of reproductive years. Secondarily, this study is designed to investigate the relationship between nocturnal urinary melatonin levels and nocturnal urinary levels of LH, FSH, and estradiol, regardless of nighttime exposure to magnetic fields. At least 180 women will be recruited as volunteers from the Seattle metropolitan area. Eligible for participation will be any woman between the ages of 20 and 45 who is a resident of King County, who has not been previously diagnosed with breast cancer, who reports regular menstrual periods, who is not taking oral contraceptives or hormone preparations, and who normally sleeps with an electric blanket during the winter months. This investigation will employ a cross-over design which treats each participant as her own control. Data collection will take place during the winter months, when an electric blanket would typically be used and will involve a three month period for each subject. The first month is designed to evaluate menstrual cycle regularity. The second and third months are each comprised of a washout period followed by either an exposure period (electric blanket use beginning the day of ovulation through Day 7 post ovulation) or a non- exposure period (no electric blanket use beginning the day of ovulation through Day 7 post evaluation). Half the subjects will be assigned to exposure followed by non-exposure while the other half of the subjects will be assigned the reverse order, non-exposure followed by exposure. Using commercial kits, ovulation will be determined for each month. During Months 2 and 3, magnetic field levels will be characterized in each subject's sleeping environment from Day 3 post ovulation through Day 7 post ovulation, and nocturnal urine will be collected on Day 7 post ovulation. Urine samples will be assayed for the primary urinary metabolite of melatonin, 6-sulphatoxymelatonin, and levels of LH, FSH, and estradiol. Information on the subject's medical history, medication use, and characteristics of the electric blanket will also be collected.